慢性低氧对大鼠血浆腺苷水平的影响  

Effect of Chronic Hypoxia on Endogenous Adenosine Concentration in Plasma of Rats

作　　者：王波[1] 谭建新[1] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001

出　　处：《实用儿科临床杂志》2012年第1期16-18,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省自然科学基金(8152402301000010)

摘　　要：目的研究慢性低氧时大鼠血浆腺苷水平的变化及其机制。方法将42只雄性SD大鼠随机分成常氧组(对照组),低氧1 d、3 d、5 d、7 d、14 d、21 d组(H1、H3、H5、H7、H14、H21组),每组6只。分别于低氧第1天、第3天、第5天、第7天、第14天及第21天测定各组大鼠血浆腺苷水平、腺苷脱氨酶(ADA)活性以及血浆中5'-核苷酸酶(5'-NT)水平。应用SPSS 16.0软件进行统计学分析。结果低氧1 d后大鼠血浆腺苷水平开始升高,于第3天达到最高峰,与对照组比较差异有统计学意义(P<0.001);此后,随着低氧时间的延长,血浆腺苷水平逐渐下降,第14天及第21天与对照组比较差异均无统计学意义(Pa>0.05)。血浆5'-NT水平在低氧第3天增高最显著(P<0.001),在低氧3 d后逐渐下降,但仍高于对照组(P<0.001)。血浆ADA活性在慢性低氧第1天、第3天较对照组明显增高(Pa<0.001),随着低氧时间的延长,其活性稍下降,但仍明显高于对照组(P<0.001)。结论慢性低氧在早期可引起血浆腺苷水平增加,后期血浆腺苷水平逐渐降低,其变化与5'-NT水平和ADA活性有关。Objective To observe the effect of chronic hypoxia on changes in plasma adenosine concentration and its mechanism in rats. Methods Forty - two male Sprague - Dawley rats were randomly divided into normoxic group （ control group） , hypoxia groups of 1 day, 3 days,5 days,7 days, 14 days,21 days groups respectively （ H1 group, H3 group, H5 group, H7 group, H14 group, H21 group）, and 6 rats were in each group. The levels of endogenous adenosine, adenosine deaminase （ADA） activity and plasma 5＇ - nucleotidase （ 5 ＇ - NT） contents were determined. SPSS 16.0 software was used for statistical analysis. Results After hypoxia, plasma adenosine content in rat began to increase on the first day, and it reached the peak on the third day, and there was a significant difference compared with the control group （P 〈 0. 001 ）, then with the longer hypoxia time, the concentration of plasma adenosine gradually decreased, on the 14th day and the 21 th, day, compared with control group,the differences were not significant compared with the control group. 5＇ - NT plasma concentration level was significantly increased on the third day（ P 〈0. 001 ） ,and it gradually declined the longer hypoxia time ,but it was still higher than that in the control group （ P 〈 0. 001 ）. ADA activity in plasma in the early stage of chronic hypoxia significantly increased （ P 〈 0. 001 ）, with longer hypoxia time, the activity decreased slightly ,but still was higher significantly than that in the control group （P 〈 0. 001 ）. Conclusions Chronic hypoxia in an early stage can lead to an increase in plasma adenosine, and it gradually decreases in a later stage, which is associated with 5＇ - NT levels and ADA activity.

关 键 词：腺苷 低氧 腺苷脱氨酶 5’-核苷酸酶 

分 类 号：R363[医药卫生—病理学]