人膀胱癌EJ细胞株CD44基因的小RNA干扰抑制细胞侵袭功能  

Inhibition of CD44 expression by small interfering RNA to suppress the metastasis of human bladder carcinoma EJ cells

作　　者：丁明霞[1] 李翀[2] 左毅刚[1] 范祖森[2] 王剑松[1] 

机构地区：[1]昆明医学院第二附属医院泌尿外科云南省泌尿外科研究所,650101 [2]中国科学院生物物理研究所

出　　处：《现代泌尿生殖肿瘤杂志》2011年第6期345-347,共3页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：云南省科技厅高层次人才培引工程经费资助项目(200807015)

摘　　要：目的研究人膀胱癌EJ细胞株中CD44基因小RNA干扰后细胞侵袭功能的变化。方法小RNA干扰的方法抑制EJ细胞中CD44基因表达,细胞划痕实验和Boyden小室研究shCD44-EJ细胞的侵袭功能改变。结果流式细胞学检测显示shCD44-EJ细胞与CD44的单克隆抗体的结合性下降,Westernblot检测CD44蛋白的表达量减少,细胞划痕实验和Boyden小室研究结果显示shCD44-EJ细胞的侵袭能力降低约50%。结论特异性沉默CD44可抑制EJ细胞的侵袭能力。Objective To investigate the effect of CD44 targeted RNA interference （RNAi） on the metastasis of human bladder carcinoma EJ cells.Methods We transfected bladder cancer cell line EJ with well-designed CD44 siRNA.Flow cytometry and Western blot analysis were used to assess the CD44 expression.Wound healing assay and Boyden chamber were used to investigate the change of shCD44-EJ cell＇s migration.Results Both flow cytometry and Western blot analysis revealed a remarkable decrease of CD44 expression in shCD44-EJ cells,but not in pSuper-puro vector transfected cells.CD44 silencing also significantly inhibited cell migration through wound healing assay.The modified Boyden chamber assay showed similar results also.Conclusions CD44 silencing can effectively inhibit the migration of EJ cells in vitro.

关 键 词：膀胱肿瘤 EJ细胞 CD44 RNA干扰 

分 类 号：R737.14[医药卫生—肿瘤]