骨唾液酸蛋白通过整合素α_vβ_3调控ILK信号通路  

Effect of Bone Sialoprotien Through Integrin α_vβ_3 on ILK Signaling Pathway of Breast Cancer MDA-MB-231 Cells

作　　者：彭远远[1,2] 王捷[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510006 [2]广州军区广州总医院医学实验科,广州510010

出　　处：《生物技术通报》2012年第1期172-176,共5页Biotechnology Bulletin

基　　金：广东省自然科学基金项目(06104396)

摘　　要：旨在探究骨唾液酸蛋白(BSP)是否通过整合素αvβ3对整合素连接激酶(ILK)信号通路进行调控。BSP基因沉默乳腺癌MDA-MB-231细胞,流式细胞仪在细胞水平检测BSP不同水平的细胞株中整合素αvβ3的表达量。Western blotting检测磷酸化ILK水平的变化,MTT法检测细胞增殖能力。与对照组231BO-Scrambled细胞相比,BSP基因沉默组231BO-BSP27细胞中整合素αvβ3的表达水平明显下调(61.32±1.94)%(P<0.01)。整合素αvβ3鼠抗单克隆抗体(LM609)处理前的BSP基因沉默组231BO-BSP27细胞与21BO-Scrambled细胞相比,ILK磷酸化水平下调明显(39.38±1.38)%(P<0.01);LM609处理后的231BO-BSP27细胞与21BO-Scrambled细胞相比,ILK磷酸化水平下调明显(33.78±1.51)%(P<0.01)。向乳腺癌细胞231BO-scrambled和231BO-BSP27中添加LM609,MTT试验结果显示两株乳腺癌细胞的增殖能力均有降低(P<0.05)。BSP通过整合素αvβ3对乳腺癌MDA-MB-231细胞ILK信号通路进行调控,并影响细胞增殖。It was to investigate whether bone sialoprotein（BSP） through integrin αvβ3 effect on the ILK signaling pathway in breast cancer MDA-MB-231 cells.BSP-silencd MDA-MB-231 cells,flow cytometric analyzed the expression of integrin αvβ3 in breast cancer MDA-MB-231 cells.Western blotting was used to detect the phosphorylation of ILK,and the proliferation of cells was analyzed by MTT assay.Results showed that compared with control group 231BO-Scrambled cells,integrin αvβ3 expression in 231BO-BSP27 cells was significantly lower（61.32±1.94） %（P 0.01）,and expression level of ILK phosphorylation was also lower（39.38 ±1.38） %（P 0.01）.Treat LM609（alpha v beta 3 rat fight monoclonal antibody） to 231BO-Scrambled cells and 231BO-BSP27 cells respectively,expressd ILK were obviously down-regulated phosphorylation level（33.78 ±1.51）%（P 0.01）.MTT experiment indicate LM609 caused proliferation inhibition in 231BO-scrambled cells and 231BO-BSP27 cells（P 0.05）.It proved that bone sialoprotein through integrin αvβ3 affect the ILK signaling pathway in breast cancer MDA-MB-231 cells.

关 键 词：骨唾液酸蛋白BSP 整合素ΑVΒ3 ILK信号通路 

分 类 号：R737.9[医药卫生—肿瘤]