17β-雌二醇对子宫内膜癌细胞增殖及p38表达的影响  

Effect of 17 beta-estradiol on proliferation of endometrial cancer cells and p38 expression

作　　者：王美丽[1] 吴中明[1] 敖第书[1] 

机构地区：[1]遵义医学院,贵州遵义563003

出　　处：《中国妇幼保健》2012年第3期386-389,共4页Maternal and Child Health Care of China

基　　金：贵州省省长基金项目〔2005:313〕

摘　　要：目的:探讨不同浓度的17β-雌二醇(E2)对雌激素受体(ER)阴性的JEC细胞和ER阳性的Ishikawa子宫内膜腺癌细胞系细胞的增殖及细胞内p38蛋白表达的影响。方法:选用人子宫内膜腺癌细胞系JEC和Ishikawa进行体外培养,加入含不同浓度E2培养液,以四甲基偶氮唑蓝(MTT)比色法和激光共聚焦扫描显微镜(CLSM)的方法,观察子宫内膜腺癌细胞系JEC和Ishikawa细胞的增殖活性及P-p38的表达。结果:①MTT法观察细胞增殖:10-7、10-8和10-9 mol/L E2作用JEC细胞4、5天后OD值与对照组比较有差异(P<0.05),其中10-7 mol/L E2组在第5天OD值与对照组比较差异较显著(P<0.01);而不同浓度E2作用Ishikawa细胞均能促进增殖(P<0.05)。②共聚焦显微镜测定细胞内P-p38蛋白的表达:10-7 mol/L的E2作用JEC 4、5天后可使细胞内P-p38表达增加,平均荧光强度值与对照组相比有统计学意义(P<0.05);10-7 mol/L的E2作用Ishikawa 3、4天后P-p38表达增加,与不加E2对照组比较差异有统计学意义(P<0.05)。结论:子宫内膜腺癌细胞系JEC细胞和Ishikawa细胞均可受到雌激素的调控,一定浓度的雌激素能促进ER阴性JEC细胞和ER阳性的Ishikawa细胞增殖,且均能使细胞内P-p38表达增加。Objective：To explore the effects of different concentrations of 17 beta-estradiol on proliferations of estrogen receptor negative JEC cells,estrogen receptor positive Ishikawa cells and p38 expression. Methods：Human endometrial cancer JEC cells and Ishikawa cells were cultured in vitro,then they were treated with culture solutions containing different concentrations of 17 beta-estradiol,MTT colorimetric method and CLSM were used to observe the proliferative activities of endometrial cancer JEC cells and Ishikawa cells and p38 expression. Results：There was significant difference in OD values of JEC cells after treated with 10-7 mol/L,10-8 mol/L,and 10-9 mol/L of 17 beta-estradiol for four and five days between observation group and control group（P0.05）.There was significant difference in OD value of JEC cells after treated with 10-7 mol/L for five days between observation group and control group（P0.01）.Different concentrations of 17 beta-estradiol could promote the proliferation of Ishikawa cells（P0.05）.10-7 mol/L of 17 beta-estradiol increased p38 expressions in JEC cells after four and five days,there was significant difference in mean fluorescence intensity between observation group and control group（P0.05）;p38 expression increased after treating Ishikawa cells with 10-7 mol/L of 17 beta-estradiol for three and four days,there was significant difference between observation group and control group（P0.05）. Conclusion：Human endometrial cancer JEC cells and Ishikawa cells both are regulated by estrogen,a certain concentration of estrogen can promote the proliferations of estrogen receptor negative JEC cells and estrogen receptor positive Ishikawa cells,and it can increase p38 expression.

关 键 词：雌激素 JEC ISHIKAWA P38 

分 类 号：R737.33[医药卫生—肿瘤]