microRNA-34a的表达对膀胱癌细胞系J82生物学行为的影响  

The Influence of ectogenesis expression of miR-34aon cellular biological behaviours in bladder cancer cell line J82

作　　者：姚志勇[1] 张超[1] 马鑫[1] 朱鸣阳[1] 张瑜[1] 史涛平[1] 杨素霞[1] 张旭[1] 

机构地区：[1]中国人民解放军总医院泌尿外科,北京100853

出　　处：《临床泌尿外科杂志》2012年第1期64-67,共4页Journal of Clinical Urology

基　　金：国家自然科学基金(No:30972982)

摘　　要：目的:探讨microRNA-34a(miR-34a)的外源性表达对人膀胱癌J82细胞生物学行为的影响。方法:将miR-34a表达质粒或对照质粒转染J82细胞,实时定量逆转录聚合酶链反应(qRT-PCR)检测miR-34a在转染细胞中的表达水平,细胞计数法、流式细胞术、Transwell侵袭实验分别检测外源性miR-34a表达后J82细胞增殖、凋亡、周期和侵袭能力的变化情况。结果:miR-34a表达质粒组与对照组比较,其miR-34a表达诱导性升高(P=0.002 6),细胞增殖受到抑制(P=0.000 1),细胞凋亡率增加(1.237%±0.116%vs 5.497%±0.293%;P<0.000 1),细胞周期阻滞于G0～G1期(54.510%±1.501%vs 62.200%±0.754%;P=0.001 5);细胞侵袭能力降低,(65.01±11.79)vs(34.33±9.71),P=0.025 4。结论:在膀胱癌J82细胞中增加miR-34a的表达,可抑制细胞增殖和侵袭,促进细胞凋亡和周期阻滞,并通过影响上述细胞生物学行为降低肿瘤细胞恶性度,miR-34a在膀胱癌细胞系中发挥潜在的抑癌基因作用。Objective：To investigate the effect of ectogenesis expression of miR-34a on cellular biological be- haviours in bladder cancer cell line J82. Method： After the transfection of miR-34a vector, the expression levels of miR-34a inJ82 cells were tested by qRT-PCR. Cell count was used to evaluate the effect of miR-34a expression on cell proliferation. Flow cytometry had been done to analyse apoptosis and cell cycle. The effect of miR-S4a expres- sion on cell invasion was assessed by transwell invasion assay. Result： Expressive levels of miR-34a were highly in duced after the transfection of miR-34a（P= 0. 002 6）. Meanwhile, we observed that miR-34a restoration inhibited cellular proliferation （P=0.000 ]）,promoted cell apoptosis （1.237%±0. 116% vs 5. 497% ±0. 293% ; P 0. 0001 ） , induced cell cycle arrest at G0 -- G1 （ 54. 510 % ±1. 501% vs62.200%±0.754%;P 0.001 5）,and re- duced cell invasion ability ,（65.01±11.79） vs （34.33±9.71）, P =0. 025 4. Conclilsion：The forced expression of miR-34a in bladder cancer cell line J82 inhibits cell malignancy by interfering with cellular biological behaviours such as proliferation,apoptosis,cell cycle and invasion, miR-34a might act as a potential tumor suppressor in blad- der cancer cells.

关 键 词：膀胱癌 增殖 凋亡 侵袭 MIR-34A 

分 类 号：R737.14[医药卫生—肿瘤]