解磷菌株B25的筛选、鉴定及其解磷能力  

Screening, identification, and phosphate-solubilizing capability of phosphate-solubilizing bacterial strain B25

作　　者：贺梦醒[1] 高毅[1] 胡正雪[1] 徐瑶[1] 龙瑞[1] 孙庆业[1] 

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《应用生态学报》2012年第1期235-239,共5页Chinese Journal of Applied Ecology

基　　金：国家高技术研究发展计划项目(2006AA06Z359);安徽省自然科学基金项目(070415208)资助

摘　　要：从安徽省铜陵市铜官山尾矿库木贼根际分离筛选出多株解磷细菌,经过多次筛选纯化获得一株解磷能力较好的菌株B25.采用透射电镜观察和DNA分子技术,确定此菌株属于芽孢杆菌属.研究了解磷菌株B25在培养168h内的解磷能力、溶液pH值以及菌株生长量的变化情况,并比较了B25在不同条件下的解磷能力.结果表明:解磷菌株B25的解磷能力与溶液pH值之间存在微弱的相关性,在碳源为葡萄糖、初始pH值为7.0、培养温度为30℃时解磷效果较好.Various phosphate-solubilizing bacterial strains were isolated from the Hippochaete ramosissimum rhizosphere in Tongguanshan copper tailings in Tongling of Anhui Province, East China. After many times of screening and purification, a strain B25 with stronger phosphate-solubilizing capability was obtained, which belonged to Bacillus genus, as identified by transmission electron microscope and DNA molecular approaches. A culture experiment was conducted to study the phosphate-solubilizing capability of the B25 within 168 h and the variations of the medium pH and B25 growth as well as the phosphate-solubilizing capability of B25 under different culture conditions. A weak correlation was observed between the phosphate-solubilizing capability of B25 and the medium pH. The B25 displayed a better phosphate-solubilizing capability when the carbon source was glucose, medium initial pH was 7.0, and culture temperature was 30 ℃.

关 键 词：解磷菌 解磷能力 16S RRNA 

分 类 号：S144.9[农业科学—肥料学]