溶血凝脂酸受体表达与卵巢癌转移的关系  

Correlation Between Lysophosphatidic Acid Receptor Expression and Ovarian Cancer Metastasis

作　　者：袁毅翀[1] 陈慧君[1] 张帆[2] 

机构地区：[1]湖北省妇幼保健院妇科,湖北武汉430070 [2]武汉大学中南医院妇瘤科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2012年第1期38-42,77,共6页Medical Journal of Wuhan University

基　　金：湖北省科技厅资助项目(编号:SJ201105)

摘　　要：目的:研究溶血凝脂酸受体(LPAR)的表达与卵巢癌转移的关系。方法:小室细胞侵袭试验检测13种卵巢癌细胞的转移能力;实时定量RT-PCR检测各细胞株、10例正常卵巢组织、32例上皮性卵巢癌中LPAR1、LPAR2及LPAR3mRNA的表达。结果:有7种卵巢细胞可被LPA诱导迁移,为侵袭性细胞;另6种对LPA反应差,为非侵袭性细胞。侵袭性细胞中均有LPAR1的表达,显著高于非侵袭性细胞(P=0.003),LPAR2及LPAR3表达与癌细胞的侵袭能力无相关性。3种受体中,仅LPAR2在卵巢癌中的表达显著高于正常卵巢组织(P=0.030)。卵巢癌中,LPAR1的表达与临床分期有关,期别越高表达越高(P=0.032);LPAR2的表达与病理分级有关,分化越差表达越高(P=0.020)。存在广泛腹腔转移的病例均有LPAR1的表达。结论:LPAR1是介导LPA诱导的卵巢癌转移的主要受体。Objective： To investigate the correlation between the expression of lysophosphatidic acid（LPA） receptors and the metastasis of ovarian cancer.Methods： Invasion assay was used to detect the invasive ability of 13 ovarian cancer cell lines;Real-time RT-PCR was used to measure the mRNA expression levels of LPAR1,LPAR2 and LPAR3 in the 13 cell lines,10 samples of normal ovarian tissue,and 32 samples of epithelial ovarian cancer.Results： Seven ovarian cancer cell lines could be induced by LPA for cell invasion,which indicated as invasive cells;and the other 6 lines had poor response for LPA that indicated as non-invasive cell.All the invasive cell lines expressed high level of LPAR1,and the level was much higher than that of noninvasive cells（P=0.003）.No correlation was observed between LPAR2 and LPAR3 expression and the cells＇ invasive ability.Among these three receptors,only LPAR2 showed higher expression level in ovarian cancer than in normal tissue（P=0.030）.The expression of LPAR1 correlated with clinical stage of ovarian cancer： the higher of stages,the higher of expression levels（P=0.032）;the expression of LPAR2 correlated with pathologic grade： the poorer of differentiation,the higher of expression level（P=0.020）.All the cases that haD extensive abdominal metastasis showed LPAR1 expression.Conclusion： LPAR1 is the main receptor that mediates the LPA induced ovarian cancer metastasis.

关 键 词：溶血凝脂酸受体 卵巢癌 转移 

分 类 号：R737.31[医药卫生—肿瘤]