热休克蛋白在良恶性嗜铬细胞瘤中的表达及其意义  

Expressionprofile of HSP90 between benign and malignant pheochromocytoma

作　　者：盛佳雁[1] 祝宇[1] 尉青[2] 吴瑜璇[1] 计骏[3] 何竑超[1] 赵菊平[1] 沈周俊[1] 马贵[1] 徐云泽[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,上海200025 [2]上海交通大学医学院附属瑞金医院病理科,上海200025 [3]上海交通大学医学院附属瑞金医院普外科,上海200025

出　　处：《现代泌尿外科杂志》2012年第1期21-23,共3页Journal of Modern Urology

基　　金：上海市自然科学基金(No.09ZR1418500);上海市教委科研创新项目(No.11YZ58)

摘　　要：目的通过检测热休克蛋白90(HSP90)在良、恶性嗜铬细胞瘤(PC)组织中的表达情况,探讨HSP90能否成为一项用于评估嗜铬细胞瘤潜在恶性程度的有效分子标记物。方法选取1986年10月～2006年8月在我院泌尿外科行手术治疗、且有完整的临床、病理和随访资料的嗜铬细胞瘤患者存档石蜡标本38例,其中良性嗜铬细胞瘤(BP)21例,恶性嗜铬细胞瘤(MP)17例。采用免疫组化技术Envision二步法,检测良、恶性嗜铬细胞瘤中HSP90的表达情况。恶性组经首次手术确诊后随访28～179月,良性组经首次手术确诊后随访93～264月。结果在MP组中HSP90的阳性表达率为70.59%,明显高于BP组中阳性表达率28.57%,两组热休克蛋白的表达具有显著的统计学差异(P<0.05)。结论 HSP90有望成为区分嗜铬细胞瘤良恶性的一项指标。Objective To investigate whether the expression of HSP90 is associated with the malignant transition of pheochromocytoma（PCs）,so as to consider the potential role of HSP90 as a useful marker of malignant PCs.Methods Tissues obtained from 38 patients with PCs（21 benign and 17 malignant intramedullar PCs） who received surgery between Oct.1986 and Aug.2006 were examined with immunohistochemistry to detect the expressions of HHP90.All tumors showed the classical histology and the typical immunohistochemical pattern（positive immunoreactivity for vimentin,chromogranin,synaptophysin,and S100）.The follow-up was 93 to 264 months for the benign group,and 28 to 179 months for the malignant group.Results HSP90 was found in 12 of the 17 malignant（70.59%） and in 6 of the 21（28.57%） benign PCs.There existed significant difference between the malignant and the benign group（P0.05）,which indicated the up-regulation of HSP90 in malignant cells of the adrenal medulla.Conclusion HSP90 is closely related to the malignant potential of PCs.The expression of HSP90 thus represents an additional prognostic marker that may identify more aggressive tumors.

关 键 词：嗜铬细胞瘤 热休克蛋白90 免疫组化染色 

分 类 号：R737[医药卫生—肿瘤]