马铃薯干腐病菌侵染过程中切片组织细胞壁降解酶的变化  

Changes of Cell Wall Degrading Enzymes in Potato Tuber Tissue Slices Infected by Fusarium sulphureum

作　　者：杨志敏[1] 毕阳[1] 李永才[1,2] 寇宗红[1] 包改红[1] 刘成琨[1] 王毅[1] 王蒂[2] 

机构地区：[1]甘肃农业大学食品科学与工程学院,兰州730070 [2]甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,兰州730070

出　　处：《中国农业科学》2012年第1期127-134,共8页Scientia Agricultura Sinica

基　　金：甘肃省科技支撑计划项目(1011JKCA179);第48批博士后基金面上项目(20100480726);甘肃省自然科学基金项目(1107RJZA232);甘肃省干旱生境作物学重点实验室开放基金项目(GSCS-2010-08)

摘　　要：【目的】探讨干腐病菌(Fusarium sulphureum Schlechlendahl)侵染过程中马铃薯切片组织主要细胞壁降解酶(cell wall degrading enzymes,CWDEs)活性的动态变化。【方法】陇薯3号马铃薯块茎组织切片接种F.sulphureum后,不同培养天数取样测定并比较分析主要CWDEs活性变化。【结果】F.sulphureum侵染的组织均能产生多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、纤维素酶(Cx)、β-葡萄糖苷酶、多聚半乳糖醛酸反式消除酶(PGTE)、果胶甲基反式消除酶(PMTE)、果胶甲基酯酶(PME)、果胶裂解酶(PML),但PG、PMG、Cx、β-葡萄糖苷酶活性显著高于其它酶。在侵染前期(1—3 d)PMG和Cx出现高峰,PG在后期(4—6 d)活性增高,而β-葡萄糖苷酶在整个侵染过程中活性一直呈上升趋势。【结论】CWDEs是F.sulphureum侵染和扩展过程中主要的致病因子,且各种CWDEs在致病中发挥作用的时期不同。[ Objective] The dynamic changes of main cell wall degrading enzymes （CWDEs） activities in potato tuber tissue slices infected by Fusarium sulphureum were investigated. [ Method] Major CWDEs activities of potato tuber slices tissue （Longshu No.3） inoculated with F. sulphureum were detected and comparatively analyzed during different sampling period. [Result] A series of CWDEs including polygalacturonase （PG）, polymethyl-galacturonase （PMG）, carboxymethyl cellulose （Cx）, β-glucosidase, polygalacturonic acid trans-eliminase （PGTE）, pectin methyl-trans-eliminase （PMTE）, pectin methylesterase （PE） and pectate lyase were detected in infected tissue. Whereas PG, PMG, Cx, and β-glucosidase showed higher activities as compared with others. The activity of PMG and Cx kept higher during early infection period （1-3 days after infection）, but higher PG activity appeared during later infection period （4-6 days after inoculation）, but β-glucosidase showed a rising trend during the enzyme dynamics analysis. [ Conclusion] These findings suggested that CWDEs were the primary pathogenic factors, and different CWDEs might take effect at different infecting periods.

关 键 词：马铃薯 FUSARIUM sulphureum 细胞壁降解酶 侵染 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]