CIK、DC-CIK细胞杀伤胃癌MKN-28细胞的作用比较  

Comparison of the killing effect of CIK cells and DC-CIK cells of gastric MKN-28 cells

作　　者：张成磊[1] 陈耀平[2] 杨宝珍[2] 潘月英[2] 王妍柏[1] 

机构地区：[1]宁夏医科大学医学实验中心,宁夏银川750004 [2]宁夏医科大学总医院检验科,宁夏银川750004

出　　处：《宁夏医学杂志》2012年第1期7-9,1,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(NZ09111)

摘　　要：目的研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后对胃癌MKN-28细胞株的杀伤作用。方法取正常人外周血,分离出外周血单个核细胞(PBMC),加入不同的细胞因子分别诱导出DC和CIK细胞,共培养成DC-CIK细胞,以MTS法测定不同组CIK细胞对MKN-28杀伤活性。结果与单纯CIK细胞相比较,DC-CIK组细胞增殖速率明显提高(P<0.05);具有更强的杀伤胃癌MKN-28细胞株的活性(P<0.05),效靶比为20.0∶1时,DC-CIK组对MKN-28的杀伤活性为(38.02±2.06)%,>CIK组的(29.78±1.84)%,差异有统计学意义。结论 DC-CIK细胞是一种增殖活性和细胞毒作用均高于单纯CIK细胞的免疫活性细胞。Objective To study the killing effect of gastric MKN - 28 cells of cytokine - induced killer cells （CIK） co - cul- tured with dendritic ceils （DC）. Methods Peripheral blood mononuclear cells （PBMC） were isolated from healthy human, DC and CIK cells were induced by different cytokines, and they were co - cultured to produce DC - CIK cells. The killing activity of different CIK groups against MKN - 28 was determined by MTS. Results Compared with CIK,the DC - CIK presented a significantly higher proliferation （ P 〈 0.05 ）. The killing effects of gastric MKN - 28 ceils were enhanced （ P 〈 0.05 ）. The activity of cytotoxicity to MKN - 28 in DC - CIK group was 38.02% ± 2.06%, and it was stronger than that in CIK group （29.78 ±1.84% ）, respectively （E/T = 20 ： 1 ）. Conclusion DC - CIK cell is a kind of immune cell which has a higher proliferation and cytotoxicity than that in CIK cell.

关 键 词：细胞因子诱导的杀伤细胞 树突状细胞 胃癌 MKN-28 

分 类 号：R735.2[医药卫生—肿瘤]