大鼠肝星状细胞分离纯化方法的改进  

Improvement in isolation and purification of SD rat hepatic stellate cells

作　　者：范建华[1] 

机构地区：[1]上海市黄浦区中心医院消化科,200002

出　　处：《检验医学与临床》2012年第2期147-148,150,共3页Laboratory Medicine and Clinic

摘　　要：目的研究肝损伤后细胞基质的沉积及纤维化形成机制,建立一种经济、简便、可靠的分离大鼠肝星状细胞(HSC)的方法。方法用链霉蛋白酶和胶原酶灌流大鼠肝脏,Optiprep密度梯度离心分离HSC,并进行体外培养。结果细胞得率为5.0×107/鼠,活率和纯度分别为98%、96%。结论该方法简便、实用、稳定、可靠,为进一步研究HSC与肝纤维化的关系,尤其是细胞水平及分子生物学水平的研究奠定了基础。Objective To investigate the mechanism of matrix accumulation and hepatic fibrosis and to develop an economic,simple and reliable method for isolation of hepatic stellate cells（HSCs） in rats.Methods HSCs were isolated from liver of rat by perfusion with pronase and collagenase,and were separated from other cells by density gradient centrifugation with Optiprep.The purity of HSCs were cultured in vitro.Results The yield of rat HSCs was 5.0×107/per rat.The cell viability was 98% and the purity of cell was 96%.Conclusion This method for isolating and culturing HSCs is simple,practical,stable and reliable,which settles the foundation of further research of the relationship between HSCs with hepatic fibrosis,particularly the research of cellular level and molecular biology level.

关 键 词：肝星状细胞 肝纤维化 密度梯度离心 

分 类 号：R575.2[医药卫生—消化系统]