超高效液相色谱-串联质谱法测定猪肉中巴喹普林残留  

Determination of Baquiloprim Residues in Pig Muscle by UPLC-MS/MS

作　　者：刘琪[1] 李丹[1] 孙雷[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2012年第1期28-30,共3页Chinese Journal of Veterinary Drug

基　　金：国家科技支撑计划课题资助项目(2006BAD31B09)

摘　　要：建立了猪肉中巴喹普林药物残留的超高效液相色谱-串联质谱测定方法,样品用乙酸乙酯提取,吹干后用60%乙腈水(0.1%甲酸)溶解,供液相色谱-串联质谱法分析,内标法定量。结果表明:巴喹普林标准溶液在100～800μg/L范围内呈现良好线性r,2均大于0.998;方法检出限为5μg/kg,定量限为10μg/kg;巴喹普林在10～80μg/kg添加范围内的平均回收率为90.8%～108%,批内、批间RSD均小于6.9%。该方法简便快捷、灵敏度高、定性准确,能满足食品安全检测有关法规的要求。An uhral performance liquid chromatography - tandem mass spectrometry （ UPLC - MS/MS） method was established for the determination of baquiloprim residues in pig muscle. The samples were extracted by Ethyl acetate, dissolved by 60% acetonitril （0. 1% Formic acid ） and determinatied on UPLC - MS/MS After evaporation. The method was quantified by Internalstandard method. The calibration curves were linear between the peak areas and the concentrations of 20 - 800 μg/L with correlation coefficients （r2） more than 0.998. The limit of detection of baquiloprim was 5 μg/kg, and the limit of quantitation was 10 μg/kg. The average recoveries from spiked muscle at concentrations of 10 - 80 μg/kg ranged 90. 8% - 108% and the relative standard deviations of intra - and inter - batch were both less than 6.9%.

关 键 词：超高效液相色谱-串联质谱 巴喹普林 残留 猪肉 

分 类 号：S859.84[农业科学—临床兽医学]