FTY720对大鼠小肠移植慢性排斥血管硬化和细胞因子影响  

Effect of FTY720 on Angiosclerosis and Cytokine of Chronic Rejection after Small Bowel Transplantation of Rats

作　　者：张海云[1] 许荣誉[1] 庄奕煌[1] 庄建良[1] 

机构地区：[1]福建医科大学附属泉州第一医院肿瘤外科,福建泉州362000

出　　处：《临床医学工程》2012年第1期28-31,共4页Clinical Medicine & Engineering

基　　金：福建省自然科学基金资助项目(项目编号:2008J0310)

摘　　要：目的研究FTY720对同种异体大鼠小肠移植慢性排斥的作用及可能作用机制。方法以F344大鼠为供体,Lewis大鼠为受体,建立小剂量环孢霉素诱导F344-to-Lewis大鼠小肠移植慢性排斥模型。实验分为四组,Ⅰ组为对照组,n=7;Ⅱ组为FTY720组,n=8;Ⅲ组为环孢霉素A(CsA)组,n=8;Ⅳ组为FTY720+CsA组,n=8。术后八周行移植物组织病理学评分和ELISA法测定血清中移植物细胞因子IL-2,IL-4,IL-6,IL-10以及TNFα,IFNγ的表达。结果术后八周移植物组织病理学评分,Ⅱ组、Ⅳ组与对照组比较均明显改善。但是仅血管硬化评分:Ⅳ组与对照组比较,差异具有显著性(P<0.05)。术后血清(IL-10、IFNγ)与移植物(IL-10),Ⅱ组、Ⅳ组与对照组比较具有显著性差异(P<0.05)。结论单独使用FTY720或联用CsA具有改善大鼠小肠移植慢性排斥血管硬化以及促进Th1/Th2平衡趋向Th2表达的趋势的免疫保护作用。Objective To investigate the effect and possible mechanism of FTY720 on the chronic rejection （CR） after small bowel transplantation of rats. Methods Using F344 rats as donors and Lewis rats as receptors to establish the model of chronic small bowel allograft rejection of F344-to-Lewis rats. The rats were randomly divided into four groups： Ⅰ ） control group, n =7; Ⅱ） FTY720 group, n=8; Ⅲ） CsA group, n =8; Ⅳ） FTY720 ＋ CsA group, n =8. Histopathological scores and the expression of allograft cytokine 1L-2, 1L-4, IL-6, IL-10 and TNFa, IFNy in blood serum by ELISA test were evaluated 8 weeks after transplantation. Results The histopathological scores 8 weeks after transplantation in group Ⅱ and group Ⅳ improved obviously in contrast to the control group. There was significant difference in vascular sclerosis scores between group IV and control group （P 〈0.05）. Compared with control group, the expression of IL-10, IFNy and IL-10 in blood serum in group Ⅱ and group Ⅳ attained statistic significance （P 〈0.05）. Conclusion These results suggest that FTY720 and FTY720＋CsA regulate the process of chronic small bowel rejection as shown by the improvement of graft arteriosclerosis and the modulation of cytokine expression. The mechanism of FTY720 deserves further study.

关 键 词：FTY720 小肠移植 慢性排斥 细胞因子 

分 类 号：R-332[医药卫生] R392.4