创伤后应激障碍大鼠动眼神经核神经元5-羟色胺1A受体表达的变化  

The Expression of 5-HT1A Receptor in the Oculomotor Nucleus of Posttraumatic Stress Disorder Rats

作　　者：刘冬娟[1] 韩芳[2] 肖冰[1] 罗斐斐[2] 徐盟[3] 王恩华[4] 石玉秀[1,2] 

机构地区：[1]中国医科大学基础医学院电子显微学研究室,沈阳110001 [2]中国医科大学基础医学院组织胚胎学教研室,沈阳110001 [3]中国医科大学第95期临床医学七年制,沈阳110001 [4]中国医科大学病理与病理生理学研究所,沈阳110001

出　　处：《中国医科大学学报》2012年第1期1-4,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(31140060,81171282);教育部博士点基金资助项目(20092104110016)

摘　　要：目的观察创伤后应激障碍(PTSD)大鼠动眼神经核神经元5-羟色胺1A(5-HT1A)受体表达的变化。方法采用无连续单一刺激(SPS)方法,建立PTSD样大鼠SPS模型,随机分为SPS后1 d、4 d和7 d组及正常对照组。应用免疫组化、Westernblot和透射电镜技术,检测动眼神经核神经元5-HT1A受体表达的变化,观察神经元超微结构的改变。结果 SPS后大鼠动眼神经核神经元5-HT1A受体的表达高于正常对照组,尤以SPS后7 d组最为明显;神经元超微结构也发生改变。结论 SPS刺激可促进大鼠动眼神经核神经元5-HT1A受体的表达增强,进而导致神经递质紊乱,影响动眼神经核支配眼球肌的运动功能,出现相应的PTSD症状。Objective To observe the change of 5-HT1A receptor-expression in the oculomotor nucleus of posttraumatic stress disorder rats. Methods The SPS-method was used to set up the rat FTSD models. Rats were randomly divided into 1 d,4 d and 7 d groups of single prolong stress （SPS） and a normal group. Immunohistochemical and Western blot were used to detect the expression of 5-HT1A receptor in the oculomotor nucleus. The change of ultrastructure in the neuron were objected by transmission electron microscopy （TEM）. Results The expression of 5-HT1A receptor in the eculomotor nucleus neurons gradually increased on 1 d,4 d and 7 d after exposure to SPS than that of the control group,and reached the peak level at 7 d after SPS exposure. There was also a change of ultrastructure in the oculomotor nucleus neuron detected. Conclusion SPS exposure resulted in the change of 5-HT1A receptor expression in the oculomotor nucleus,which might be related to the pathogenesis of PTSD.

关 键 词：创伤后应激障碍 动眼神经核 5-羟色胺1A受体 神经元 免疫组化 

分 类 号：R749.5[医药卫生—神经病学与精神病学]