17β-雌二醇对高氧诱导的鼠视网膜新生血管形成的影响  

Effects of 17β-estradiol on the oxygen-induced retinal neovasularization in rat

作　　者：马晓华 毕宏生 李镜海 解孝锋 季鹏 吴建峰 

机构地区：[1]山东施尔明眼科医院,济南250002

出　　处：《中华眼底病杂志》2012年第1期62-67,共6页Chinese Journal of Ocular Fundus Diseases

基　　金：基金项目：山东省科技攻关计划项目（2008GG30002031）

摘　　要：目的观察并探讨17β-雌二醇对高氧诱导的鼠视网膜新生血管形成的影响及机制。方法48只新生Sprague—Dawley（SD）大鼠随机分为对照组和实验组，每组各24只。对照组大鼠分娩完成后与新生鼠一起正常饲养；实验组大鼠分娩完成后立即与新生鼠一起置于高氧环境饲养。对照组和实验组又再分为磷酸盐缓冲液（PBS）干预组（对照组1、实验1）和雌二醇干预组（对照组2、实验组2），每组各12只大鼠。对照组1和实验组1大鼠分别每日皮下注射PBS0．1ml；对照组2和实验组2大鼠分别每日皮下注射雌二醇1μg。每日观察鼠的发育情况。出生后7、14d逆转录聚合酶链反应（RT-PCR）检测视网膜血管内皮生长因子（VEGF）、低氧诱导因子-1α（HIF-1 α）mRNA的含量。出生后14d时行苏木精-伊红（HE）染色观察视网膜新生血管生长情况；免疫组织化学染色观察VEGF蛋白的表达；透射电子显微镜观察视网膜超微结构变化。结果出生后14d各组大鼠标本中突破内界膜的内皮细胞数比较，差异有统计学意义（F=10．7，P〈0．05）。其中，实验组1较对照组1明显增多，差异有统计学意义（q=4．28，P〈0．05）。实验组2较实验组1明显减少，差异有统计学意义（q=5．16，P〈0．05）。实验组2与对照组1比较，差异无统计学意义（q=-0．25，P〉0．05）。出生后14d各组大鼠VEGF蛋白表达比较，差异有统计学意义（F=10．7，P〈0．05）。其中，实验组1与对照组、实验组2比较，差异有统计学意义（q=5．41，4．35，P〈0．05）。视网膜超微结构显示，实验组1神经节细胞肿胀，细胞质淡染，线粒体空泡形成；其他各组视网膜超微结构正常。出生后7、14d各组大鼠视网膜VEGF、HIF-1mRNA表达比较，差异均有统计学意义（F=14．7，16．1，13．4，17．5；P=0．001，0．005，0．003，0．009）。其中，出生后7d，对照组2VEGF表Objective To investigate the effects neovasularization in rats with oxygen-induced retinopathy and mechanism of 17β-estradiol on the retinal （OIR）. Methods A total of 48 SD rats were randomly divided into control group A, control group B, experimental group A and experimental group B with 12 rats in each group. The rats in control group A and experimental group A received a hypodermic injection of 0. 1 ml PBS, and the rats in control group B and experimental group B group received an a hypodermic injection of 0. 1 ml 17β-estradiol. At postnatal day 7 （P7） and P14, the mRNA expression of vascular endothelial growth factor （VEGF） and Hypoxia-inducible factor （HIF） 1 α in the retina were measured by real-time potymerase chain reaction （RT-PCR）. At P14, endothelial cell nuclei breaking through the internal limiting membrane were counted after staining with hematoxylin and eosin （HE）, and the protein expression of VEGF was measured after immunohistochemical staining. The changes of retinal ultrastructure were observed by transmission electron microscopy. Results At P14, the difference of the number of endothelial cell nuclei among four groups was statistically significant （F= 10.7, P〈0.05）. The number of endothelial cell nuclei in experimental group A was increased greater than that in control group A （P〈0.05） and experimental group B（q=5.16,P〈0.05）. But there was no difference between control group A and experimental group B （q=0.25 ,P〉0.05）. The difference of VEGF protein expression among the four groups was statistically significant （P〈0.05）. Comparing experimental group A with control group A, B and experimental group B, the difference was statistically significant （P〈0.05）. In experimental group A there was ganglion cell swelling, pale staining cytoplasm and mitochondria vacuolizationin, while these were normal in other three groups. At P7 and P14, the differences of VEGF and HIF-1 mRNA expression among four groups were statistically significant （F=

关 键 词：视网膜新生血管化/病因学 雌二醇 血管内皮生长因子类 缺氧诱导因子1 Α亚基 

分 类 号：R774.1[医药卫生—眼科]