眼前节内眼模拟手术诱发血眼屏障破坏的大鼠动物模型  

Rat model of blood-ocular barrier breakdown induced by anterior segment intraocular analogic surgery

作　　者：谢茂松[1] 郑永征[2] 徐国兴[1] 

机构地区：[1]福建医科大学附属第一医院眼科福建省眼科研究所,福州350005 [2]福建中医药大学附属人民医院眼科

出　　处：《中华眼底病杂志》2012年第1期68-72,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：基金项目：福建省自然科学基金（2009J05063）;福建省科技平台建设项目（2010Y2003）

摘　　要：目的建立眼前节内眼模拟手术诱发血眼屏障破坏的大鼠动物模型。方法清洁级健康成年雄性Sprague—Dawley大鼠150只，随机分为对照组和模型组，每组75只。按1ml／kg的剂量腹腔注射盐酸氯胺酮一盐酸甲苯噻嗪混合液麻醉大鼠。磷酸盐缓冲液灌注袋连接三通管。三通管一端连接24G静脉留置针，手术显微镜下在3点时钟位从角巩缘前透明角膜30。斜行穿刺入前房，退出针头，留置套管；另一端连接24G静脉留置针套管，与测压计相连测量大鼠眼压。大鼠眼压波动于0～12mmHg（1mmHg=0．133kPa）之间，波动30次／min，重复60次。采用氧氟沙星滴眼液滴眼。建模后第1、2、3、5、7天，采用免疫组织化学法检测大鼠白蛋白；定量检测大鼠房水、视网膜中伊凡思蓝（EB）浓度。结果免疫组织化学染色结果显示，建模后第1、2、3、5、7天对照组白蛋白阳性染色均局限于虹膜和视网膜血管内，脉络膜弥漫性着色。建模后第1天，模型组白蛋白阳性染色主要位于虹膜和视网膜神经层血管周围；建模后第2、3天，阳性染色扩散到虹膜和视网膜全层；建模后第5、7天，阳性染色主要局限于虹膜和视网膜血管内。模型组房水中EB浓度在建模后第1、2、3、5天，均较对照组高（t=25．781，37．433，25．150，19．171；P〈0．01）；建模后第7天，与对照组接近（t=1．303，P=0．209）。模型组视网膜EB浓度在建模后第1、2、3天，均较对照组高（t=11．997，14．622，23．014；P〈0．01）；建模后第5、7天，与对照组接近（t=2．027，O．756；P=0．058，0．459）。结论通过模拟眼前节内眼手术损伤因素，可建立内眼手术诱发的血眼屏障破坏的大鼠动物模型。Objective To establish a rat model of blood ocular barrier breakdown induced by anterior segment intraocular analogic surgery. Methods One hundred and fifty healthy adult male rats were randomly divided into control group and model group, 75 rats in each group. The rats were anesthetized with 1 ml/kg ketamine hydrochloride/xylazine hydrochloride solution. Three way pipes were attached to a phosphate buffer infusion bag and two intravenous catheters. One catheter was inserted 30° obliquely through the transparent cornea anterior to the limbus into the rat＇s anterior chamber. Then the needle was withdrawn and the sheath was indwelling. Another catheter was connected with a manometer. Intraocular pressure was varied from 0 to 12 mm Hg （1 mm Hg=0. 133 kPa） 60 times, 30 times per min. The catheter was removed. The eyes were treated with ofloxacin ophthalmic solution after surgery. The 1st, 2nd, 3rd, 5th and 7th day after surgery, the integrity of the blood ocular barrier was assessed by immunohistochemical staining for albumin and quantitative measurement using Evanr s blue as a tracer. Results Albumin immunohistoehemical staining of the control group was confined to the iris and retinal blood vessels. The choroid was stained at each time point after surgery. Albumin immunohistochemieal staining of the model group was abundant around the iris and the retinal vasculature on the 1st day after surgery. The albumin diffused throughout the iris and the retina on the 2nd and the 3rd day after surgery. The albumin reached the retinal vessels on the 5th and 7th day after surgery. The aqueous humor Evans blue leakages of the model group were higher than those of the control group on the 1st, 2nd, 3rd and 5th day after surgery. The differences were statistically significant （t=25.781, 37. 433, 25. 150, 19. 171; P〈0.01）. The Evans blueleakage of the model group was close to that of the control group on the 7th day after surgery. The difference was no statistical significant（t= 1. 303, P 0. 209）. The retinal Evan

关 键 词：血房水屏障 动物实验 

分 类 号：R-332[医药卫生] R779.6