高同型半胱氨酸血症对孕鼠胚胎发育的影响  

Influence of Hyperhomocysteinemia on Embryo Development of Pregnant Rats

作　　者：卢艳[1] 王新[2] 王海琴[3] 

机构地区：[1]湖南省妇幼保健院综合治疗科,湖南长沙410008 [2]济南市第五人民医院妇产科,山东济南250022 [3]中南大学湘雅二医院妇产科,湖南长沙410011

出　　处：《医学临床研究》2011年第12期2324-2326,共3页Journal of Clinical Research

摘　　要：[目的]探讨高同型半胱氨酸血症（Hhe）对大鼠妊娠的影响.[方法]将30只SD孕鼠按同型半胱氨酸（HCY）浓度高低随机分为 高剂量组（H组）、低剂量组（L组）、对照组（C组）,每组10只.从妊娠d7起,H组腹腔内注 HCY 200 mg/（kg·d）,L组注射100 mg/（kg·d）,C组腹腔内注射同等体积的生理盐水,直至孕d20剖宫取胎.计算各组仔鼠数,死胎数,测定仔鼠体重、身长,用高效液相色谱电化学方法检测妊娠前及妊娠d20孕鼠血浆HCY浓度.[结果]①H组、L组与C组孕鼠妊娠d20血浆CHY浓度[分别为（30.47±1.12）,（20.90±1.08）,（10.98±0.77 ） μmol/L]比较差异有统计学意义（均P〈0.05）.②随着HCY剂量的增加,H组和L组仔鼠数下降,仔鼠的体重、身长降低,死胎率及致畸率增加与C组比较差异均有统计学意义（均P〈0.05）.且H组与L组之间差异亦有统计学意义（P〈0.05）.[结论]Hhe对孕鼠胚胎发育有毒性作用,且存在量效关系,主要表现为仔鼠数减少、仔鼠生长受限、畸形、死胎.[Objective] To explore the influence of hyperhomocysteinemia（HHE） on rat pregnancy. [Methods]According to the level of homocysteine（HCY）, 30 SD pregnant rats were randomly divided into high dose group（group H）, low dose group（group L） and control group（group C） with 10 in each. From d7 to d20 of pregnancy before uterine-incision delivery, group A was intraperitoneally injected with 200mg/（kg. d）, and group L was injected with 100mg/（kg. d）, and group C was injected the equal volume of normal saline. The number of young rats and the dead fetus were counted. The weight and the height of young rats were measured. High performance liquid chromatography and electrochemical method were used to detect plasma HCY levels of pregnant rats before and d20 of pregnancy. [Results]Plasma HCY levels of pregnant rats in group H, L and C at d20 of pregnancy were 30.47±1.12, 20.90±1.08 and 10.98±0.77umol/L respectively, which were higher than those before the pregnancy, and there were significant differences among groups （all P 〈0.01）. As the dose of HCY increased, the number, weight and height of young rats in group H and L decreased, and the rate of dead and malformation fetus increased. There were significant differences between group H or L and group C（all P 〈0.05）. There was also significant difference between group H and group L （ P 〈0.05）. [Conclusion] HHE may exert the toxic effect on embryo development of pregnant rats and there is dose-effect relationship, which mainly shows the decreasing of the number of young rats, growth retardation, malformation and dead fetus.

关 键 词：高同种半胱氨酸血症 胎儿发育 疾病模型 动物 大鼠 

分 类 号：R589.3[医药卫生—内分泌]