产琥珀酸菌的快速筛选及鉴定  

Rapid isolation and identification of a succinic acid producing strain

作　　者：林日辉[1] 黄新林[1] 黄文勤 谭秋培[1] 孙丹[1] 

机构地区：[1]广西民族大学化学与生态工程学院化学与生物转化过程新技术广西高校重点实验室,南宁530006 [2]南宁奕德环境科技有限公司,南宁530003

出　　处：《食品科技》2012年第1期13-16,共4页Food Science and Technology

基　　金：国家民委科研项目(10GX07);广西教育厅科研项目(201012MS072);广西民族大学重点项目科研基金资助课题(200701YJ01)

摘　　要：琥珀酸是重要的化工原料,广泛应用于食品、医药等领域。采用简单、高效的筛选方法,从牛瘤胃内容物分离获得1株有潜力的琥珀酸生产菌。通过生理生化分析和16SrDNA同源性比对,鉴定为琥珀酸放线杆菌(Actinobacillus succinogenes)。Succinic acid is an important chemical for used widely in the fields of food, Pharmaceutical industry. This paper designed a convenient and effective method for the screening of succinic acid producer from bovine rumen, and a potential strain was isolated. By the physiological and biochemical determination, and the 16S rDNA molecular analysis, it was determined that the strain was belong to Actinobacillus succinogenes.

关 键 词：琥珀酸发酵 ACTINOBACILLUS succinogenes 菌种鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]