转化生长因子β_1及白介素-1β对终板软骨细胞的作用  

Experimental study with transforming growth factor β_1 and interleukin-1β for Cartilage endplate cells

作　　者：田峰[1] 崔学生[1] 张帅[1] 石玉泽[1] 金群华[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学附属医院骨科,银川750004

出　　处：《重庆医学》2012年第1期36-37,39,I0001,共4页Chongqing medicine

基　　金：宁夏科技攻关基金资助项目(2008)

摘　　要：目的研究转化生长因子β1(TGF-β1)及白介素-1β(IL-1β)在终板软骨细胞中的表达,探讨椎间盘退变的发病机制。方法应用免疫组化SP染色法检测TGF-β1及IL-1β在20例颈椎病及20例非颈椎病椎体软骨终板中的表达,并对其阳性表达结果进行比较。结果颈椎病组TGF-β1及IL-1β的阳性率分别为(32.907 6±3.076 4)%、(51.520 7±2.192 5)%,与非颈椎病终板软骨细胞TGF-β1及IL-1β表达的阳性率(42.481 6±2.061 7)%、(33.918 5±3.320 4)%相比,差异有统计学意义(P<0.05)。结论 TGF-β1及IL-1β参与了椎间盘退变的形成和发展,在椎间盘退变的发病中可能起重要的作用。Objective To investigate the influence of transforming growth factor beta 1（TGF-β1） and interleukin 1 beta（IL-1β） on human cartilage endplate.To preliminarily probe the action mechanism of TGF-β1 and IL-1β in the occurrence and evolution of intervertebral disc degeneration and to provide novel pathway for preventing and curing intervertebral disc degeneration.Methods Monitor the expression of TGF-β1 and IL-1β of centrum cartilage endplate gathered from November 2009 to June 2010 in our hospital by immunohistochemistry（IHC）.The lesion group consists of 20 centrum cartilage endplates resectionlied in the anterior surgery of cervical spondylosis and the control group is comprised of 20 centrum cartilage endplates gathered in the anterior surgery of cervical fracture.Results The positive rate of TGF-β1 and IL-1β in the lesion group was（32.907 6± 3.076 4）% and（51.520 7±2.192 5）%,respectively;while the positive rate of TGF-β1 and IL-1β in the control group was（42.481 6±2.061 7）% and（33.918 5±3.320 4）%,respectively.The overall positive expression of TGF-β1 in the control group was significantly higher than that in the lesion group,whereas the overall positive expression of IL-1β in the lesion group was significantly higher than that in the control group,and both of the differences were distinct enough to have a statistical implication（P〈0.01）.Conclusion The expression TGF-β1 and IL-1β existed in degenerated human cartilage endplate,and the expression of TGF-β1 and IL-1β in the lesion group was lower and higher than their counterparts in the control group,respectively.The positive stain of TGF-β1 and IL-1β were mainly located in the cytoplasm and cytomembrane of cartilage cells,and also appeared in the nucleus.The qualitative and quantitative results of expression of TGF-β1 and IL-1β obtained by IHC and western blot are in good accordance,thus providing reference for research and clinical work.

关 键 词：转化生长因子Β1 椎间盘 退变 软骨细胞 白细胞介素1Β 

分 类 号：R363[医药卫生—病理学]