梨花中熊果苷的高效液相色谱分析  

Quantification of Arbutin from Pear Blossoms by HPLC

作　　者：李媛[1] 乔进春[1] 张玉星[1] 何童森[1] 吕明霞[1] 张飞[1] 崔同[1] 

机构地区：[1]河北农业大学食品科技学院,保定071000

出　　处：《天然产物研究与开发》2012年第1期76-78,83,共4页Natural Product Research and Development

摘　　要：采用高效液相色谱法对不同时期、不同品种的梨花中熊果苷的含量进行了分析,色谱柱为Hypersil BDSC18(4.6 mm×250 mm,5μm),流动相为甲醇∶水(6∶94),加入甲酸0.05%,检测波长280 nm。结果表明:熊果苷在0.01～5.00μg范围内线性关系良好(r=0.9999),平均加标回收率为97.7%。不同生长期的鸭梨梨花中熊果苷的含量一般在10 mg/g以上,尤以花芽萌动期时含量最高,达到35.7 mg/g(鲜重计)。不同品种的9份梨花样品中,熊果苷含量在3.5～10.5 mg/g之间。The contents of arbutin in pear blossoms of different periods and different varieties were detected using the HPLC method with Hypersil BDS C18 column（4.6 mm × 250 mm,5 μm）,the mobile phase methanol ∶ water（6∶ 94,with 0.05% formic acid）,and the detection wavelength 280 nm.The results showed that the calibration curve was linear in the range of 0.01~5.00 μg（r = 0.9999） and the average recovery was 97.7%.The content of arbutin in Yali pear blossom during the florescence was above 10 mg/g（fresh weight） in most cases,especially in blossom bud germination period,reaching a maximum of 35.7 mg/g.In the nine pear blossom samples from different varieties,the contents of arbutin were range between 3.5 mg/g to 10.5 mg/g.

关 键 词：梨花 熊果苷 HPLC 

分 类 号：R284.2[医药卫生—中药学]