肠血管活性肽对大鼠急性胰腺炎肠微循环及炎性介质的影响  

Protective effect of VIP on intestinal microcirculation and inflammatory mediates of acute necrotizing pancreatitis in rats

作　　者：龚红生[1] 王崇高[1] 毛正发[2] 

机构地区：[1]南京市第一医院浦口分院(浦口区中心医院)普外科,211800 [2]江苏大学附属医院,212001

出　　处：《疑难病杂志》2012年第1期40-42,F0003,共4页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨小剂量肠血管活性肽(VIP)对急性坏死性胰腺炎(ANP)大鼠肠组织10血流量及炎性介质的影响。方法 SD大鼠96只,随机分为对照组(C组)和治疗组(T组),以5%牛磺胆酸钠于胰腺被膜下均匀注射复制ANP模型。T组经尾静脉持续滴注VIP(0.2 pmol·g^(-1)·h^(-1),持续12h),C组按同样方法注射等量生理盐水。采用放射性生物微球技术在制模后2h及12h分别测定肠组织血流量,同时检测血清TNF-α及IL-1β水平,并观察肠黏膜病理改变。结果与C组比较,T组在制模后2h及12h,肠组织血流量均明显增加(P<0.05),血清TNF-α、IL-1β水平均明显降低(P<0.05,P<0.01),肠黏膜组织损伤程度明显减轻(P<0.01)。结论在ANP早期,VIP能降低血清TNF-α及IL-1β的水平,增加肠道微循环血流量,减轻肠黏膜炎性反应。Objective To explore the protective effect of vasoactive intestinal peptide（VIP） on the changes of intestine injury,intestinal microcirculation and inflammatory mediators in acute necrotizing pancreatitis（ANP）.Methods A total of 96 rats were randomized into control group（C group） and treatment group（T group）.The model of ANP was established by injection of 5%sodium taurocholate into the pancreatic capsule.Little dosage of VIP（0.2 pmol ？ g^（-1）？h^（-1） injected from tail veins of T group,equation dosage of NS into C group.Radioative biomicrosphere technique was applied to measure the intestinal blood flow at 2h,12h after ANP.Simultaneously,blood sample and intestine tissues were collected to observe the changes of TNK-α,IL-1βby histological exam.Results Compared with C group,the intestinal blood flow in T group increased hig her at 2h,12h after ANP（P0.05） was established.But the levels of TFN-α,IL-1βin serum were decreased（P0.05, P0.01）.The intestinal pathological changes in T group were alleviated highly than that in the C group（P0.01）.Conclusion In the early stage of ANP,the VIP could decrease the levels of TFN-αand IL-1βin serum,increase the intestinal blood flow,and release the inflammation in intestinal mucosa.

关 键 词：肠损害 微循环 肠血管活性肽 胰腺炎 坏死性 急性 大鼠 

分 类 号：R576[医药卫生—消化系统]