多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜的制备及降解性能  

Preparation and degradation property of composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate

作　　者：吴岳恒[1] 张灵敏[2] 曾祥君[3] 麦丽萍[1] 武长礼[3] 汤顺清[2] 余细勇[1] 

机构地区：[1]广东省医学科学院广东省人民医院医学研究中心,广东省广州市510080 [2]暨南大学生物医学工程所,广东省广州市510632 [3]广东省医学科学院广东省心血管病研究所心外科,广东省广州市510080

出　　处：《中国组织工程研究与临床康复》2011年第51期9531-9534,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30901468),课题名称:偏磷酸钙微粒改善多孔聚四羟丁酸膜细胞亲和性的实验研究~~

摘　　要：背景：种子细胞能否成功黏附于支架材料表面,与支架材料的降解性能密切相关。目的：制备多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜,并观察其体外降解性能。方法：将聚3-羟基丁酸-4-羟基丁酸和偏磷酸钙按50∶50混合均匀,完全溶解于氯仿后,搅拌均匀,倾倒在培养皿上,干燥后即成多孔膜。将其置于循环装置中,流速为0.30m/s,管路内灌满37℃生理盐水50mL。结果与结论：流场作用多孔复合膜2,4,8,16,24h后,其失重率分别为4.5%,5.6%,6.9%,9.6%和10.6%;扫描电镜显示,降解24h后,表面偏磷酸钙微粒已降解完全或脱落,而内凹偏磷酸钙微粒仍嵌入在聚3-羟基丁酸-4-羟基丁酸复合膜内,复合膜表面直径2μm的微孔基本不见,直径30~50μm的大孔基本未变,但孔隙边缘变得圆滑;水静力称重法计算出降解实验前后复合膜吸水率、显气孔率和密度分别为降解前的40.6%,46.3%,1.14g/cm3和降解后的51.6%,54.1%1.05g/cm3。说明实验制备的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜降解早期以偏磷酸钙降解为主,降解过程中孔内微丝易降解或断裂。BACKGROUND： Degradation properties of scaffold are closely related to the adhering of seed cells to its surface. OBJECTIVE： To prepare the composite membrane of porous poly （3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate （P （3HB-co-4HB）/CMP） and observe its degradation properties in vitro. METHODS： The raw material ratio of P （3HB-co-4HB）/CMP was 50/50, and the P （3HB-co-4HB）/CMP was mixed uniformly and dissolved in chloroform and dumped in a Petri dish. After drying, the porous composite membranes were obtained. The porous composite membranes were put in the circulation device, the flow rate of which was 0.30 m/s, the channel was filled with 50 mL 37 ℃ physiological saline. RESULTS AND CONCLUSION： Weight loss rates of the composite membrane at 2, 4, 8, 16, 24 hours under flow field were 4.5%, 5.6%, 6.9%, 9.6% and 10.6% respectively; scanning electron microscopy showed that the partial calcium phosphate particles on the surfaces were complete depredated or lost, the concave particles were kept, 2 μm porous on the surfaces could not be seen, 30-50 μm porous did not change but the edge was smooth; the water absorption, porosity and density of the composite membrane were 40.6%, 46.3%, and 1.14 g/cm3 respectively before depredation, and 51.6%, 54.1%, 1.05 g/cm3 after that. During the preparation of P （3HB-co-4HB）/CMP, the depredation of the composite membrane in the early stage is the partial calcium phosphate depredation, and the microfilament is prone to be depredated and facture during the depredation.

关 键 词：聚3-羟基丁酸-4-羟基丁酸 降解 偏磷酸钙 复合膜 材料 吸水率 显气孔率 密度 

分 类 号：R318[医药卫生—生物医学工程]