MBP-1分子真核表达载体的构建及其在食管癌细胞株Eca109中的高表达  

Construction of the Eukaryotic Expressing Vector for MBP-1 and Its Expression in Esophageal Cancer Cells

作　　者：李雪婷[1] 谢勇恩[1] 

机构地区：[1]川北医学院病理生理学教研室,四川南充637007

出　　处：《川北医学院学报》2011年第6期469-471,共3页Journal of North Sichuan Medical College

基　　金：四川省青年科技基金项目(08ZQ026-081)

摘　　要：目的:初步探讨MBP-1的分子功能。方法:通过PCR扩增获得MBP-1基因编码序列,将其定向插入质粒pcD-NA3.1(+)构建重组质粒pcDNA3.1-mbp-1,重组质粒转染体外培养的食管癌Eca109细胞株,Western-blotting检测MBP-1在食管癌细胞中的表达。结果:筛选出分子量较大的重组质粒,酶切分析和DNA测序分析证实重组质粒含MBP-1基因完整编码序列,重组质粒转染食管癌Eca109细胞株后呈现高表达,其表达量约为对照细胞的2.1倍。结论:成功构建了MBP-1分子真核表达载体并在食管癌细胞中实现了高表达,为进一步深入研究MBP-1的分子功能奠定了基础。Objective： To preliminarily study the molecular function of MBP-1 molecule.Methods： The MBP-1 gene fragment was obtained by polymerase chain reaction（PCR）.The MBP-1 gene was inserted into plasmid pcDNA3.1（＋） to construct the recombinant eukaryotic expressing vector for MBP-1.Esophageal cancer cells were transfected with a recombinant vector.MBP-1 expression was detected by using Western-blotting.Results： A recombinant plasmid was obtained during screening.The recombinant plasmid contained the coding sequence of MBP-1 gene,which was identified by restriction enzyme analysis and DNA sequencing.Western-blotting analysis showed that MBP-1 was overexpressed in esophageal cancer cells with recombinant plasmid transfection.Conclusions： A recombinant eukaryotic expressing vector for MBP-1 was successfully constructed.MBP-1 was over expressed in esophageal cancer cells after transfection with the recombinant plasmid.These results provide the basis for further study of the molecular function of MBP-1.

关 键 词：MBP-1 真核表达载体 食管癌细胞 

分 类 号：R730.23[医药卫生—肿瘤]