蝎素组分Ⅲ对脂多糖刺激人白血病单核细胞系THP1细胞TLR4和p65的表达  

Effect of scopion venom component Ⅲ on expression of toll like receptor 4 and p65 in lipopolysaccharide stimulated human leukemia monocytic THP-1 cells

作　　者：宋向凤[1,2] 牛杰[3] 刘玉峰[1] 

机构地区：[1]郑州大学一附院儿科,河南省郑州市450052 [2]新乡医学院免疫学教研室,河南省新乡市453003 [3]新乡医学院形态学实验室,河南省新乡市453003

出　　处：《中国组织工程研究与临床康复》2011年第49期9215-9217,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河南省教育厅基金资助项目(2010A31005)~~

摘　　要：背景:近年来研究表明,Toll样受体和核因子κB与白血病的发生发展关系密切。蝎毒对肿瘤细胞表面Toll样受体和细胞内核因子κB的影响还未见文献报道。目的:观察蝎素组分Ⅲ对人白血病单核细胞系THP1细胞Toll样受体4和p65表达的影响。方法:将THP1细胞接种于24孔细胞培养板中,实验分5组:对照组、脂多糖(终浓度为1mg/L)组及不同浓度蝎素组分Ⅲ处理组(脂多糖+蝎素组分Ⅲ终浓度为1,10,20mg/L)。Real-timePCR法检测THP1细胞Toll样受体4和p65mRNA的表达;Westernblot检测Toll样受体4和p65蛋白的表达。结果与结论:脂多糖组THP1细胞Toll样受体4、p65mRNA和蛋白的表达均显著高于对照组(P<0.01);不同浓度蝎素组分Ⅲ处理组Toll样受体4、p65mRNA和蛋白表达均低于脂多糖组(P<0.01),并呈剂量依赖性。提示蝎素组分Ⅲ能有效下调脂多糖诱导的THP1细胞中TLR4的表达,并可有效抑制核因子κB的活性,从而抑制细胞的增殖。BACKGROUND：In recent years,studies have shown that toll-like receptors and nuclear factor κB（NF-κB） have a close relation with the occurrence and development of leukemia.However,there were no reports about the effect of scorpion venom on toll-like receptors on the surface of tumor cells and NF-κB.OBJECTIVE：To investigate the effect of scopion venom component Ⅲ（SVC-Ⅲ） on expression of toll like receptor 4（TLR4） and p65 in lipopolysaccharide（LPS） stimulated human leukemia monocytic THP-1 cells.METHODS：THP1 cells were inoculated in 24-well cells culture plates,and there were five groups：control group,LPS（1 mg/L） stimulation group and SVC-Ⅲ treatment group（LPS＋ SVC-Ⅲ,1,10,20 mg/L）.Real-time PCR was employed to detect the expression of TLR4 and p65 mRNA in THP1 cells,Western blot was used to detected the expression of TLR4 and p65 protein.RESULTS AND CONCLUSION：The expression of TLR4 and p65 mRNA and protein of THP1 cells in LPS stimulation group was significantly higher than that in the control group（P〈0.01）.However,the expression of TLR4 and p65 mRNA and protein of THP1 cells in SVC-Ⅲ treatment group was lower than that in LPS stimulation group（P〈0.01） and in dose-dependent manner.SVC-Ⅲ can down-regulate the expression of TLR4 and inhibit the activity of NF-κB in LPS stimulated THP1 cells in order to inhibit the cells proliferation.

关 键 词：蝎毒 Toll样受体4 脂多糖 THP-1 核因子κB 白血病 

分 类 号：R394.2[医药卫生—医学遗传学]