RUNX3基因在肝癌组织的mRNA表达水平及其临床意义  

Expression of RUNX3 mRNA in primary liver cancer and its clinical significance

作　　者：卢燕辉[1] 李建国[2] 江小杰[2] 

机构地区：[1]解放军第一七五医院,漳州363000 [2]福建医科大学附属漳州市医院,漳州363000

出　　处：《中华肝胆外科杂志》2012年第1期30-33,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：福建省自然科学基金资助（2009J01326）

摘　　要：目的探讨RUNX3基因在肝癌组织和癌旁组织的mRNA表达水平及其与临床病理因素的相关性。方法采用RT-PCR检测RUNX3基因在51例肝癌组织及其癌旁组织的mRNA表达水平，分析RUNX3基因mRNA表达与临床病理因素的相关性。结果RUNX3基因在51例肝癌组织的mRNA表达量相对值为0．4509±0．0963；在相应癌旁组织的mRNA表达量相对值为0．9147±0．0222；两者差异有显著统计学意义（t=33．6087，P〈0．001）。RUNX3基因mRNA表达水平在分化程度、浸润周围组织、癌栓、肝内转移等病理因素方面的差异有统计学意义（P〈0．05）。在性别、肿瘤直径、肿瘤部位、癌肿出血坏死、组织分型方面的差异无统计学意义（P〉0．05）。结论RUNX3基因在肝癌组织的mRNA表达水平显著低于在癌旁组织。RUNX3基因mRNA低表达与分化程度、浸润周围组织、癌栓、肝内转移等病理因素相关。RUNX3基因可能是肝癌的一种抑癌基因。Objective To study the expression of RUNX3 mRNA in primary liver cancer （PHC） tissue and its surrounding normal tissue, and its clinical significance. Methods Reverse transcription polymerase chain reaction （RT-PCR） was performed to detect the expression of RUNX3 mRNA in tumor and peritumor tissues in 51 patients with PHC. The relationship between RUNX3 mRNA expression and some clinical pathological parameters was analyzed. Results The relative expres- sion values of RUNX3 mRNA in the tumor tissue and the surrounding normal tissue were 0. 4509±0.0963 and 0. 9147±0.0222, respectively. The difference was significant （t=33. 6087,P〈0. 001）. The RUNX3 mRNA expression in tumor tissue correlated with some clinical pathological parameters including low tumor differentiation,positive cancer embolus and intrahepatic invasion and metastasis. The RUNX3 mRNA expression was not correlated wish other clinieopathological parameters including gender, cancer diameter, cancer location, hemorrhage and necrosis of cancer, and histotype. Conclusion RUNX3 may be a new tumor suppressor gene for PHC.

关 键 词：肝癌 抑癌基因 RUNX3基因 信使核糖核酸 

分 类 号：R735.7[医药卫生—肿瘤]