膜截流分子量对新型生物人工肝支持系统内细胞材料影响的实验研究  

An experimental study on the effects of membrane molecular weight cut off on a novel bioartificial liver system

作　　者：施晓雷[1] 张悦[2] 韩冰[1] 顾劲扬[1] 褚薛慧[1] 肖江强[1] 任昊桢[1] 檀家俊[1] 丁义涛[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肝胆外科,210008 [2]南京医科大学鼓楼临床学院

出　　处：《中华肝胆外科杂志》2012年第1期46-49,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金（30772129）;江苏省自然科学基金（BK2006008）;江苏省临床医学基金（ZX200605）;南京市科技局重点课题：基于纳米支架材料的新型生物人工肝支持系统;东南大学生物电子学国家重点实验室开放研究基金资助

摘　　要：目的 探索膜截流分子量对新型生物人工肝（bioartificial liver，BAL）支持系统内细胞材料功能的影响。方法 选用200×10。和1200×10。两种不同膜截流分子量的半透膜作为BAL隔离膜。正常比格犬接受新型BAL治疗6h，定时收集BAL系统中培养液。观察各组细胞活力及功能变化。结果200×10。组细胞活力保持在90％左右，明显高于1200×10。组的22％。细胞功能检测显示，200×10^3组细胞白蛋白分泌水平及尿素合成分别为53．3μg／10。细胞和3．6μg／10。细胞，显著高于1200×10。组的5．6μg／10^6细胞和0．3gg／10^6细胞。1200×10^3组反应器内免疫球蛋白分子浓度显著高于200×10^3组，免疫荧光试验进一步证实了上述结果。结论 降低BAL膜截流分子量可以有效减少免疫球蛋白分子的透过，从而维持BAL中细胞材料的功能。Objective To study the effects of membrane molecular weight cut off on a novel bioartificial liver（BAL） system. Methods Healthy beagles underwent 6-hour treatment with a BAL containing membrane with 200 kDa retention rating or 1200 kDa retention rating. The functional changes and cell viability were characterized. Results Hepatocyte performance levels such as albumin secretion, urea synthesis and viability were significantly higher in the 200 kDa retention rating group when compared with the 1200 kDa retention rating group （P〈0.05）. Significant levels of canine proteins were detected in the BAL medium from the 1200 kDa retention rating group. Fluorescence microscopy further verified that heavy deposition of canine IgG, IgM and complement （C3） on co-culture cells were obtained after BAL treatment in the 1200 kDa retention rating group. Conclusions Small membrane molecular weight cut off of BAL could reduce the transfer of xenoreactive antibodies into the BAL medium and improved the performance of the BAL.

关 键 词：生物人工肝 膜截流分子量 肝细胞 

分 类 号：R575.3[医药卫生—消化系统]