强化训练对脑缺血再灌注大鼠臂板蛋白3A及其受体神经纤毛蛋白-1表达的影响  

Effects of intensive training on the expressions of semaphorin 3A and neuropilin-1 after cerebral ischemia- reperfusion in rats

作　　者：王强[1,2] 王佩佩[2] 孟萍萍[2] 岳寿伟[1] 

机构地区：[1]山东大学齐鲁医院康复医学科,济南250012 [2]青岛大学医学院附属医院康复医学科

出　　处：《中华物理医学与康复杂志》2012年第1期2-7,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：山东省教育厅科研发展计划（J09LF22）

摘　　要：目的观察不同强度运动训练对脑缺血再灌注大鼠脑内臂板蛋白3A（Sema3A）及其受体神经纤毛蛋白-1（NP．1）的表达以及缺血侧脑细胞凋亡的影响，探讨强化运动训练促进脑缺血再灌注大鼠运动功能恢复的可能机制。方法采用线栓法建立左侧大脑中动脉阻塞（MCAO）2h再灌注动物模型。60只造模成功的雄性Wistar大鼠按随机数字表法分为训练1组、训练2组、训练3组、对照组（各15只鼠）。训练1组大鼠每天游泳1次，每次5min；训练2组大鼠每天游泳1次，每次10min；训练3组大鼠每天游泳2次，每次10min；对照组大鼠不做任何训练；另15只鼠为假手术组不阻塞大脑中动脉血流，不训练。采用Garcia神经功能缺损评分评价神经功能缺损情况。采用TUNEL染色观察缺血区大脑皮质细胞凋亡情况。应用免疫组织化学法对脑缺血后脑内神经生长抑制因子Sema3A及其受体NP-1的表达进行分析。结果假手术组神经行为功能正常。对照组各时间点的Garcia神经行为功能评分与假手术组同时间点比较，差异均有统计学意义（P〈0．01）；各训练组游泳训练7和14d后的Garcia神经功能评分明显优于同时间点对照组（P〈0．01），且训练3组神经功能评分提高最明显，其游泳训练3、7和14d后的Garcia神经功能评分分别为（12．80±0．45）、（15．204-0．45）和（16．80-4-0．45）分。游泳训练3、7和14d后，训练1组、训练2组、训练3组的Sema3A、NP-l及TUNEL各指标阳性细胞的表达均明显低于对照组相同时间点（P〈0．01），以训练3组更为明显。训练3组游泳训练3、7和14d后，TUNEL阳性细胞率分别为（29．43±1．38）％、（22．30±1．21）％和（17．58±1．70）％；Sema3A阳性细胞率分别为（19．64±1．17）％、（9．73-4-3．83）％和（8．24-t-O．87）％；NP-1阳性细胞率分别为（33．95-4-6．86）％、（27．95±1．29）％和（18．[Abstract] Objective To observe the effects of intensive training at different intensities on the expressions of semaphorin 3A （ Sema 3A） and its receptor neuropilin （ NP-1 ） and the cell apoptosis in cerebrum after cerebral is- cbemia-reperfusion in rats, and to investigate the possible mechanism of intensive training in recovery of motor func- tion after cerebral ischemia-reperfusion in rats. Methods To establish animal model of cerebral ischemia-reperfu- sion in rats, the intraluminal thread method was applied to cause left middle cerebral artery occlusion （MCAO） for 2 h and before reperfusion. After cerebral ischemia-reperfusion model were established for 24 h, 60 male model Wist- ar rats were randomly divided into training group I （ swimming for 5 min once a day） , training group 2 （ swimming for 10 min once a day） , training group 3 （swimming for 10 rain twice a day） and control group （no training） ; another 15 rats assigned to the sham-operation group were subject to no MCAO and no training. Neurological function was e- valuated by Garcia scores, and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate-biotin nick end labeling （TUNEL） was performed to dectect the cortical cell apoptosis. Expressions of neural growth inhibition factor Sema 3A and its receptor NP-1 were detected by immunohistochemistry. Results The neurological function in sham-operation group was normal. The differences of Garcia scores at different time points beween sham-operationgroup and control group were significant （P 〈 0.01 ）. Garcia scores in all training groups, were significantly higher than those in controls at the 7th and ldth d after swimming training （P 〈 O. O1 ）, especially in training group 3 the Garcia scores were （ 12.80 ±O. 45 ）, （ 15.20 ± 0.45 ）, （ 16.80 ±0.45 ）, respectively, at the 3rd, 7th and 14th d af- ter swimming training. The rates of positive cell of Sema 3A, NP-1 and TUNEL indexes in all training groups were lower than those in cont

关 键 词：脑缺血再灌注 强化训练 臂板蛋白3A 神经纤毛蛋白-1 细胞凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]