前列腺癌组织中miRNAs的表达及意义  

Expression of miRNAs in prostate cancer and its significance

作　　者：江燕[1] 李明[1] 尹玉[1] 曹立宇[1] 李昊[1] 徐晓春[1,2] 张洪福[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]美国UTM．D．AndesonCancerCenter

出　　处：《临床与实验病理学杂志》2012年第1期50-53,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的研究前列腺癌组织中6种miRNAs的表达及其意义,并探讨它们在前列腺癌诊断中的应用价值。方法采用核酸分子原位杂交(in situ hybridizationI,SH)技术,结合组织微阵列平台分别检测38例良性前列腺增生(benign prostate hyperpla-sia,BPH)和52例前列腺癌(prostatic cancer,PCa)中6种miRNAs的表达情况。结果 6种miRNAs在PCa中的表达率与BPH比较差异均有统计学意义(P<0.05)。6种miRNAs的表达均与PCa的Gleason评分相关(P<0.05);与患者的年龄及血清PSA水平均无明显相关性(P>0.05)。miR-15b、miR-182和miR-96的表达与PCa的临床分期相关(P<0.05);miR-96的表达与肿瘤累及前列腺叶数相关(P<0.05)。结论 miRNAs与PCa的发生、发展以及生物学行为有关,并可能作为PCa诊断及判断预后的生物标记物。Purpose To investigate the expression of 6 individual miRNAs and their significance in prostate cancer, and to discuss their use for the diagnosis of prostate cancer. Methods In situ hybridization staining combined with tissue microarray technology for 6 miRNAs was performed in 38 samples of benign prostate hyperplasia and 52 samples of prostate cancer. Results The expression of all 6 miRNAs showed a significant difference between the prostate cancer and benign prostatic hyperplasia tissues （ P 〈 0. 05 ）. The positive rate of these 6 miRNAs was significantly related to the Gleason grade of prostate cancer（ P 〈 0. 05 ） , but not with age and serous PSA level of the patient （P 〉 0. 05 ）. On the otherhand, the expression of miR-15b , miR-182 and miR-96 correlated with tumor clinical stage （ P 〈 0. 05 ）, The expression of miR-96 correlated with the extension of tumor invasion in prostate （ P 〈 0. 05 ）. Conclusions The miRNAs may be involved in the development of prostate cancer, and they may be important biomarkers for diagnosis and prognostic assessment of nrostate cancer.

关 键 词：前列腺肿瘤 前列腺增生 MIRNAS 原位杂交 

分 类 号：R737.25[医药卫生—肿瘤]