经动脉灌注端粒酶抑制剂AZT对大鼠Walker-256肝肿瘤的影响  

Effects of intraarterial infusion of telomerase inhibitor AZT on walker-256 liver cancer xenograft in rats

作　　者：张火俊[1] 陆建平[1] 杨继金[1] 朱俊 李欲晓[1] 郝强[1] 生晶[1] 张顺民[4] 

机构地区：[1]第二军医大学附属长海医院放射科,上海200433 [2]江苏省如皋市城西医院 [3]长海医院 [4]第二军医大学附属长海医院病理科

出　　处：《放射学实践》2012年第1期17-20,共4页Radiologic Practice

基　　金：国家自然科学基金(81171435);上海科委自然基金(10ZR1438200);国家留学回国人员科研启动基金(教外司留20101174)部分资助

摘　　要：目的:观察经动脉灌注端粒酶抑制剂AZT对大鼠种植型walker-256肝癌的影响,探讨采用经动脉介入法应用端粒酶抑制剂治疗肝脏恶性肿瘤的可能性。方法:36例walker-256荷瘤大鼠分为两组,AZT组大鼠在显微镜下经肝动脉灌注端粒酶抑制剂AZT,对照组大鼠采用同样方法灌注等量生理盐水。7天后分别测定各组肿瘤组织端粒酶活性、细胞凋亡率及肿瘤体积的变化。结果:两组大鼠处理前端粒酶活性差异无统计学意义,处理后第7天AZT组肿瘤组织端粒酶活性(A值)明显低于对照组(AZT组0.384±0.131、对照组0.739±0.156,P<0.05),而肿瘤细胞凋亡率与对照组比较则明显升高[AZT组(14.218±0.913)%、对照组(4.025±0.287)%,P<0.05],两组肿瘤体积比较AZT组明显小于对照组[AZT组(371.665±25.298)mm3、对照组(772.943±52.221)mm3,P<0.05]。结论:经动脉灌注AZT能明显降低大鼠肝脏walker-256肿瘤组织的端粒酶活性、提高肿瘤细胞凋亡率、抑制肿瘤生长,采用经动脉灌注端粒酶抑制剂治疗肝脏恶性肿瘤是一种可能应用于临床并值得进一步深入研究的新方法。Objective：To observe the effect of intraarterial infusion with telomerase inhibitor ATZ on xenograft walker-256 liver cancer in rats,in order to investigate the possibility of intraarterial infusion of telomerase inhibitor on the treatment of liver cancer.Methods：The telomerase inhibitor（azidothymidine,AZT） was infused into liver cancer（walker-256） xenograft in SD rats.The influence on telomerase activity,apoptotic indices（AI） and tumor volumes were detected and evaluated.Telomerase activity was examined by telomeric repeat amplification proctocol coupled with ELISA（TRAP-ELISA）.Morphological changes were observed under microscopy,AI and tumor volumes were calculated respectively.Results：The tumor volume was reduced magnificantly in AZT group than that in control group（treated with normal saline）.The A value of telomerase activity in control group and AZT group were 0.739±0.156,0.384±0.131（P0.05）,AI of two groups were（4.025±0.287）%,（14.218±0.913）% respectively（P0.05）.Conclusion：Intraarterial infusion of AZT can decrease telomerase activity,induce apoptosis and reduce tumor growth rate.AZT infusion is a promising treatment for malignant liver tumors.

关 键 词：端粒酶 端粒酶抑制剂 肝肿瘤 大鼠 放射学 介入性 

分 类 号：R735.7[医药卫生—肿瘤] R815[医药卫生—临床医学]