5-氮杂-2′-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响  

Effects of 5-Aza-2′-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2

作　　者：刘瑶[1] 贺兴波[2] 谢军[1] 孟凡[1] 杨建琼[3] 黄才斌[1] 

机构地区：[1]赣南医学院第一附属医院消化内科,江西赣州341000 [2]赣南医学院第一附属医院医务科,江西赣州341000 [3]赣南医学院第一附属医院临床医学研究中心,江西赣州341000

出　　处：《肿瘤防治研究》2012年第1期9-12,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(81000908);江西省教育厅科技项目(GJJ11210)

摘　　要：目的探讨去甲基化药物5-氮杂-2′-脱氧胞苷(5-Aza-CdR)对人肝癌细胞系HepG2凋亡及其PEG10基因表达调控的影响,进一步探讨肝癌的发病机制及5-Aza-CdR治疗肝癌的可行性。方法用浓度为50μmol/L的5-Aza-CdR分别处理HepG2细胞24、48、72 h,hoechst 33342/PI双染荧光显微镜检测HepG2细胞凋亡情况。RT-PCR、Western blot法分别检测PEG10 mRNA和蛋白表达水平的变化。结果与对照组相比,5-Aza-CdR作用24、48、72 h后,HepG2细胞的凋亡率明显升高,PEG10 mR-NA和蛋白表达水平显著下降,并呈现时间依赖性(P<0.05)。结论 5-Aza-CdR可促进HepG2细胞凋亡并抑制PEG10基因的表达。Objective To investigate the effects of demethyhfing agent 5＇-Aza 2＇-deoxyeytidine （5-Aza- CdR）on the expression of PEG10 gene in human hepato carcinoma cell line HepG2. Methods HepG2 cells were treated with 50. 1） μmol/L 5-Aza-CdR for 24,48,72 h. Apoptosis of HepG2 induced by 5-Aza- CdR was identified by fluorescence microscopy with hoechst 33342/PI staining. RT-PCR and Western blot were applied to detect the PEGI0 mRNA and protein expression levels,respectively. Results After treated with 5-Aza-CdRat for indicated time, fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells increased significantly,and the expression of PEG10 mRNA and protein in HepG2 cells were reduced in a time-dependent manner（P〈0.05）. Conclusion 5-Aza-CdR can induce HepG2 cell apoptosis and reduce the expression of PEG10 gene.

关 键 词：5-氮杂-2′-脱氧胞苷 PEG10 凋亡 HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]