慢性复合应激对食管肿瘤大鼠模型细胞免疫及肿瘤标志物的影响  

Effects of Chronic Composite Stress on Cell Immunity and Tumor Marker in Esophageal Neoplasia Model Rats

作　　者：王继云[1] 张俊权[1] 张建伟[1] 王建军[1] 刘本刚[1] 李万刚[1] 

机构地区：[1]中国石油天然气集团公司中心医院胸外科,河北廊坊065000

出　　处：《肿瘤防治研究》2012年第1期28-31,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究慢性复合应激对食管肿瘤大鼠的细胞免疫和肿瘤标志物的影响。方法选用Wistar大鼠,制作食管肿瘤大鼠模型,造模成功后,将40只食管肿瘤大鼠随机分为两组,每组20例,一组行4周慢性复合应激,一组正常饲养。观察实验组与对照组应激前后及组间各指标变化,分析慢性复合应激对大鼠细胞免疫功能及肿瘤标志物的影响。结果 40只患食管肿瘤的Wistar大鼠实验前的CD3+、CD4+、CD4+/CD8+值分别为(65.37±4.51)μg/L、(30.68±5.40)μg/L、(1.37±0.22)μg/L,实验组应激后值分别为(82.67±5.55)μg/L、(17.81±1.53)μg/L、(0.84±0.12)μg/L,与应激前比较差异有统计学意义(P<0.05)。对照组(67.14±1.49)μg/L、(35.70±5.72)μg/L、(1.31±0.15)μg/L,与实验前接近,差异无统计学意义(P>0.05)。实验组与对照组比较差异有统计学意义(P<0.01)。肿瘤标志物实验前CEA的含量为(4.85±0.93)μg/L,SCC的含量为(1.82±0.61)μg/L,实验组应激后CEA和SCC分别为(6.82±1.06)μg/L和(3.12±1.21)μg/L,与实验前比较差异有统计学意义(P<0.05),与对照组比较差异亦有统计学意义。其余指标差异均无统计学意义(P>0.05)。结论慢性复合应激导致肿瘤大鼠细胞免疫力下降,对肿瘤进展有一定影响。Objective To study the effect of multiple stresses on the changes of cellular immunity and tumor marker in oesophageal neoplasia of rats by animal modeling. Methods Wistar rats were chosen for experiment and pathological examination fully confirmed the occurrence of oesophageal cancer in the examined rats. After success of modeling,40 rats with oesophageal neoplasia were randomly divided into two groups,with twenty in each group. One group were treated with chronic composite stress. The other group was used as control,and normally feed. Before the start of experiment,T-lymphocyte subsets,i, e. CD3 ＋ ,CD4 ＋ , CD4 ＋/CD8 ＋ and tumor marker, i. e. CEA, CA19-9, CA724, CYFRA21-1 and SCC in each rat were measured. Four weeks stress was supplied for treated rats. Then above parameters were measured again in the control and treated rats. The data were analyzed between control and treated groups. Therefore, we could conclude the effects of stress on cellular immunity function and tumor markers. Results Values of CD3 ＋ ,CD4 ＋ ,CD4 ＋/CD8 ＋ of 40 rats with oesophageal neoplasia before experiment were respectively （65.37 ± 4.51）μg/L, （30.68 -±5.40） μg/L and （1.37± 0.22）μg/L,while they were （82.67 ± 5.55） μg/L, （17.81±1.53）μg/L and （0. 84±0. 12） μg/L of the treated group after the stress. There were statistic significance before stress and after stress （P〈0.05）. In contrast values of the control were （67.14 ± 1.49） μg/L, （35.70 ±5.72）μg/L and （1.31 ± 0.15） μg/L after experiment,with- out statistic significance （P^0.05）. There were statistic significance between the control and treatment （P0.01）. Contents of tumor marker CEA and SCC before experiment were （4.85± 0.93） μg/L and （1.82 ± 0.61） μg/L,and after experiment for treated group were （6.82 ± 1.06） μg/L and （3.12 ± 1.21）μg/L with statistic significances compared with the control and before experiment, respectively （P 0.05）. Other three parameters for tumor marker w

关 键 词：应激 食管肿瘤 大鼠 细胞免疫 肿瘤标志物 

分 类 号：R735.1[医药卫生—肿瘤]