B细胞特异性莫洛尼鼠白血病病毒插入位点-1在胃癌组织中的表达及其临床意义  

B-cell-specific Moloney murine leukemia virus integration site-1 expression in gastric carcinoma and its clinical significance

作　　者：刘朋伟[1,2] 邢敬[1,2] 林云[1,2] 彭延申[1,2] 陈晓宇[1,2] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科 [2]上海市消化疾病研究所消化病理室,上海200001

出　　处：《肿瘤》2012年第1期49-55,共7页Tumor

基　　金：上海市科学技术委员会科研计划项目(编号:09DZ1950101)

摘　　要：目的:探讨B细胞特异性莫洛尼鼠白血病病毒插入位点-1(B-cell-specific Moloney murine leukemia virus integration site-1,Bmi-1)蛋白在胃癌组织中的表达及其临床意义。方法:应用免疫组织化学法检测正常胃黏膜、慢性非萎缩性胃炎、慢性萎缩性胃炎、异型增生及胃癌组织中Bmi-1、c-myc和Zeste基因增强子同源物2(enhancer of Zeste homolog 2,EZH2)蛋白的表达。结果:Bmi-1蛋白在正常胃黏膜、慢性非萎缩性胃炎、慢性萎缩性胃炎、异型增生和胃癌组织中的阳性表达率分别为5.0%、16.7%、30.7%、58.1%和78.0%,各组间差异有统计学意义(P<0.05)。胃癌组织中Bmi-1高表达率为45.0%,其表达水平与肿瘤大小、分化程度、浸润深度、淋巴结转移和TNM分期有关(P<0.05)。胃癌组织中Bmi-1的表达与c-myc、EZH2表达之间呈正相关(rs=0.567,P<0.001;rs=0.582,P<0.001)。结论:Bmi-1在胃癌组织中表达上调,其高表达与胃癌的分化程度、浸润深度和淋巴结转移有关。Objective： To investigate the expression of B-cell-specific Moloney murine leukemia virus integration site-1 （Bmi-1） in gastric carcinoma and its clinical significance. Methods： The expressions of Bmi-1, c-myc, and enhancer of Zeste homolog 2 （EZH2） in normal gastric mucosa, chronic non-atrophic gastritis, chronic atrophic gastritis, dysplasia and gastric adenocarcinoma tissues were detected by immunohistochemistry. Results： The positive expression rates of Bmi-1 in normal gastric mucosa, chronic non-atrophic gastritis, chronic atrophic gastritis, dysplasia and gastric carcinoma tissues were 5.0%, 16.7%, 30.7%, 58.1% and 78.0%, respectively. There was a significant difference in positive expression rate of Bmi-1 among different gastric tissues （P〈O.05）. The high expression rate of Bmi-1 （45.0%） in gastric adenocarcinoma tissues was associated with tumor size, differentiation, invasion depth, lymph node metastasis and TNM stage （P〈0.05）. The Bmi-1 expression was positively correlated with the expressions of c-myc （rs = 0.567, P〈0.001） and EZH2 （rs = 0.582, P〈0.001）. Conclusion： The expression level of Bmi-1 is up-regulated in gastric carcinoma, and which is related with differentiation, invasion depth and lymph node metastasis.

关 键 词：胃肿瘤 基因表达 免疫组织化学 B细胞特异性莫洛氏鼠白血病病毒插入位点-1 

分 类 号：R735.2[医药卫生—肿瘤]