HDAC6与P21^(WAF1)在胃癌组织中表达及意义  

Expression and Clinical Significance of HDAC6 and P21^(WAF1) in Gastric Carcinoma

作　　者：王秀茹[1] 刘文天[2] 张秋瓒[3] 乔丽葵[3] 张淑贤[3] 

机构地区：[1]天津医科大学总医院,在读研究生天津市300052 [2]天津医科大学总医院消化科 [3]天津医科大学第四中心临床学院

出　　处：《中国肿瘤临床》2012年第1期5-9,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨HDAC6与P21^(WAF1)在胃癌发生、发展中的作用及其相关性,为胃癌的早期诊断、治疗和判断预后提供依据。方法:应用SP免疫组化法分别检测40例胃癌、30例癌前病变组及32例正常胃黏膜组中H)AC6及P21^(WAF1)蛋白的阳性表达情况。结果:HDAC6组织表达阳性率在胃癌,癌前病变及正常对照组依次降低,分别为65.0%,43.3%,15.6%(P<0.01),P21^(WAF1)组织表达阳性率在胃癌,癌前病变及正常对照组依次升高,分别为45.0%,63.3%和81.3%(P<0.01)。HDAC6的表达在分化程度越低、分期越高和淋巴结转移胃癌组织中显著升高(P<0.05);P21^(WAF1)蛋白阳性表达则相反,在分化程度越低、分期越高和淋巴结转移胃癌组织中显著降低(P<0.05)。胃癌组织中HDAC6与P21^(WAF1)的表达呈负相关(r-0.495,P<0.01);癌前病变、黏膜组织中HDAC6与P21^(WAF1)的表达呈负相关(r=-0.451,P<0.05)。结论:HDAC6与P21WAF1的异常表达可能与促进胃癌的发生、发展和浸润转移有关。HDAC6可能通过下调P21^(WAF1)的表达,导致并促进胃癌的发生发展。HDAC6和P21^(WAF1)可能作为胃癌诊断和预后判断的指标及胃癌治疗的新靶点。Objective： The current paper aims to investigate the HDAC6 and p21^WAF1 expression, as well as their correlations, in gastric carcinoma and normal mucosa adjacent to carcinoma, The roles of these proteins in gastric carcinoma were also investigated. Methods： Immunohistochemistry was performed to detect the HDAC6 and p21^WAF1 expression in 40 specimens of gastric carcinoma, 30 specimens of precancerous lesions （ chronic atrophic gastritis with intestinal metaplasia ）, and 32 specimens of normal mucosa adjacent to carcinoma. Results： The expression of HDAC6 in gastric carcinoma, precancerous lesions, and normal mucosa was 65.0%, 43.3%, and 12.5%, respectively. Meanwhile, the positive rates of p21^WAF1 in gastric carcinoma, precancerous lesions, and normal mucosa were 45.0%, 63.3%, and 81.3%, respectively. A significant difference among the expression of HDAC6 and p21^WAF1 in the three groups （ P 〈 0.01 ） was observed. In gastric carcinoma, the HDAC6 expression significantly increased in stage III or IV tumors （ P 〈 0.05 ）, positive lymph-node metastasis, and poorly differentiated adenocarcinoma （ P 〈 0.05 ）. On the contrary, the p21^WAF1 expression significantly de- creased in stage III or IV tumors （ P 〈 0.05 ）, positive lymph-node metastasis, and poorly differentiated adenocarcinoma （ P 〈 0.05 ）. A negative correlation was found between the HDAC6 and p21 p21^WAF1 expression in gastric carcinoma （ r = -0.495; P 〈 0.01 ） and precancerous lesions （ r = -0.451; P 〈 0.05 ）. Conclusion： HDAC6 may have an inhibitory effect on p21^WAF1 and may promote the development, infiltration, and metastasis of gastric carcinoma. HDAC6 and p21^WAF1 are possible molecular markers of gastric cancer and may be used in the diagnosis and protmosis and as theraneutic targets of gastric carcinoma.

关 键 词：HDAC6 P21~WAF1 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]