蛋白激酶Cθ在胃肠道间质肿瘤中的表达及意义  

PKC θ expression in gastrointestinal stromal tumors

作　　者：杨晓东[1] 王志强[1] 董令仪[1] 张辉[1] 叶颖江[1] 王杉[1] 

机构地区：[1]北京大学人民医院胃肠外科外科肿瘤研究室,100044

出　　处：《中华普通外科杂志》2012年第1期34-36,共3页Chinese Journal of General Surgery

摘　　要：目的 研究蛋白激酶C θ（protein kinase C θ,PKC θ）在胃肠道间质瘤（gastrointestinal stromal tumors,GIST）组织中的表达,并评估其作为GIST鉴别诊断标志物的价值.方法 采用免疫组织化学染色法检测121例GIST肿瘤组织及51例配对瘤旁间质组织和70例非GIST间叶源性肿瘤组织中PKC θ的表达情况,并分析其与GIST临床病理特征之间的关系. 结果 本组GIST肿瘤组织中PKC θ的阳性表达率为91.7％,其中包括8例CD117阴性GIST,而51例GIST瘤旁间质组织及26例平滑肌肿瘤中均无PKC θ表达,其他胃肠道间叶源性肿瘤中PKC θ阳性表达率为18.2％.PKC θ的表达与GIST的部位、细胞形态、恶性风险程度分级无关.结论 PKC θ可能作为GIST,尤其是CD117阴性的GIST鉴别诊断的辅助标志物.Objective To evaluate protein kinase Cθ（PKCθ）for the differential diagnosis of gastrointestinal stromal tumors（GIST）.Methods The expression pattern of PKC θ was detected by immunohistochemistry in 121 GISTs,paired 51 distant normal tissues and 70 non-GIST sarcomas.Results Immunoreactivity for PKC θ was detected in 111 of 121 GIST cases,all eight CDll7-negative GISTs were PKC θ positive.PKC θ was negative in all distant normal tissue samples and 26 smooth muscle tumors.The positive expression rate of gastrointestinal mesenchemal tumors other than GIST was 18.2%.The expression rate of PKC θ did not vary with of tumor location,cell morphology and risk grades.Conclusions PKC θ could be a potential diagnotic marker in GIST patients,especially in those with negative CD117.

关 键 词：胃肠道间质肿瘤 蛋白激酶C 肿瘤标记物 生物学 

分 类 号：R735[医药卫生—肿瘤]