癌胚抗原阴性的人大肠癌组织蛋白质组的研究  

Proteomic profiling of CEA-negative human colorectal carcinoma

作　　者：陈远光[1] 李娟云[1] 梁剑荣[2] 廖东江[2] 卢心鹏[2] 柯传锋[1] 胡明[1] 雷建[1] 

机构地区：[1]广州医学院第一附属医院胃肠外科,广东广州510120 [2]广州呼吸疾病研究所国家重点实验室,广东广州510120

出　　处：《广州医学院学报》2011年第4期1-4,共4页Academic Journal of Guangzhou Medical College

基　　金：广州医学院基金资助项目03-Q-06;广东省自然科学基金资助项目04300798

摘　　要：目的:比较癌胚抗原(CEA)阴性的人大肠癌组织与CEA阳性的人大肠癌组织之间的蛋白质表达差异。方法:收集大肠癌患者切除癌组织的新鲜标本,根据血清CEA分为阴性组、阳性组,同时取正常黏膜作对照组,提取各组组织的总蛋白,进行双向凝胶电泳和凝胶图谱差异分析,比较各组组织之间表达差异点,将这些差异蛋白点进行质谱分析数据库鉴定。结果:各组双向电泳胶上蛋白斑点图象清晰,阴性组和阳性组中差异表达的蛋白点18个,成功鉴定其中10个为9种蛋白质。其中,在阴性组中特异表达的蛋白质点为含波形蛋白的混合物1、肝脂肪酸结合蛋白,表达上调的蛋白质点为肌动蛋白结合蛋白22-α、热休克蛋白27序列、血红蛋白β链突变体;在阳性组中特异表达的蛋白质点磷酸甘油醛异构酶、角蛋白10、角蛋白1,表达上调的蛋白质点为β微管蛋白链。结论:CEA阴性的人大肠癌组织与CEA阳性的人大肠癌组织存在蛋白质组差异表达,这些蛋白可能与大肠癌表达CEA有关。Objective：To evaluate the proteomic differences among the CEA negative and positive human colorectal carcinoma tissues. Methods：Fresh human colorecta] carcinoma tissues were collected and classified into CEA-negative group and CEA-positive group according to the serum CEA level. Tissues of normal mueosa were enrolled as control group. Proteins in the three groups was separated, and then processed by two- dimensional gel electrophoresis （2-DE）. Spots with differential expression were identified by mass spectrometry. Results： Distinct images of protein spots on 2-DE gel in each group were obtained. There were 18 differentially expressed protein spots in CEA negative and positive groups, 10 of which were successfully identified to be of 9 types of protein. In CEA-negative group, spots of vimentin-containing mixture I and liver fatty acid-binding protein were differentially expressed, and those of actin binding protein 22-a, HSP27 sequences and mutant hemoglobin beta chain were upregulated. In CEA positive group, spots of triosephosphate isomerase, keratin 10 and keratin 1 were differentially expressed, and those of tubulin beta chain were upregulated. Conclusion： Differentially expressed proteins probably correlated with CEA expression were identified between CEA negative and positive human colorectal carcinoma tissues.

关 键 词：大肠癌 癌胚抗原 蛋白质组学 

分 类 号：R735.3[医药卫生—肿瘤]