Oxidative stress regulated heme-oxygenase-1 and glutathione S-transferase-m1 gene expression changes in cell lines exposed to melanins  

作　　者：Jie Li Peng Zhao Junfeng Yang Renyun Zhang Shen Li Dan Liu 

机构地区：[1]Department of Psychiatry, Tianjin Medical University, Tianjin 300070, China [2]Department of Neurology, General Hospital of Tianjin Medical University, Tianjin 300052, China

出　　处：《Neural Regeneration Research》2011年第34期2661-2665,共5页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China, No. 30600201;the Tianjin Science and Technology Committee, No. 07JCYBJC09900

摘　　要：To investigate the effects of oxidative stress on substantia nigra neuronal degeneration and death in patients with Parkinson＇s disease, we treated neuroblastoma cells （SK-N-SH） and glioma cells with Fenton＇s reagent, iron chelating agent, neuromelanin and dopamine melanin. We investigated the changes in expression of nine oxidative stress-related genes and proteins. The levels of mRNAs for heme-oxygenase-1 and glutathione S-transferase-ml were significantly reduced in SK-N-SH cells exposed to oxidative stress, and increased in glial cells treated with deferoxamine. These results revealed that SK-N-SH neurons react sensitively to oxidative stress, which implies different outcomes between these two types of cells in the substantia nigra. Moreover, the influences of neuromelanin and dopamine melanin on cell function are varied, and dopamine melanin is not a good model for neuromelanin.To investigate the effects of oxidative stress on substantia nigra neuronal degeneration and death in patients with Parkinson＇s disease, we treated neuroblastoma cells （SK-N-SH） and glioma cells with Fenton＇s reagent, iron chelating agent, neuromelanin and dopamine melanin. We investigated the changes in expression of nine oxidative stress-related genes and proteins. The levels of mRNAs for heme-oxygenase-1 and glutathione S-transferase-ml were significantly reduced in SK-N-SH cells exposed to oxidative stress, and increased in glial cells treated with deferoxamine. These results revealed that SK-N-SH neurons react sensitively to oxidative stress, which implies different outcomes between these two types of cells in the substantia nigra. Moreover, the influences of neuromelanin and dopamine melanin on cell function are varied, and dopamine melanin is not a good model for neuromelanin.

关 键 词：human neuromelanin dopamine melanin gene expression heme-oxygenase-1 glutathione S-transferase-ml oxidative stress neurons glial cells 

分 类 号：Q55[生物学—生物化学] TQ926.1[轻工技术与工程—发酵工程]