间歇牵拉应变对大鼠骨髓间充质干细胞增殖与成骨分化的影响  

Effect of intermittent tensile strain on proliferation and osteogenic differentiation of rat bone mesenchymal stem cells

作　　者：吴玉琼[1] 房兵[1] 江凌勇[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院,正颌-正畸治疗中心,上海市口腔医学重点实验室,上海200011

出　　处：《医用生物力学》2011年第6期574-579,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目资助项目(30901698,10972142);上海市科学技术委员会资助(08411961600)

摘　　要：目的探讨体外间歇拉伸应变对大鼠骨髓间充质干细胞(rat bone mesenchymal stem cell,rBMSC)的增殖与成骨分化效应。方法应用Flexcell-4 000应力系统,将间歇拉伸应变(振幅10%,频率0.5 Hz,每天作用2次,4 h/次)作用于体外分离培养的正常rBMSC,加力1、3、5、7 d后检测应力对细胞形态改变、细胞增殖及骨形成核心结合因子Cbfα1、碱性磷酸酶(ALP)和I型胶原等成骨基因和Cbfα1蛋白的影响。结果 rBMSC在间歇牵拉应力作用1 d后即出现增殖速度减缓,一直延续到加力第7 d,ALP和I型胶原等成骨基因的表达自加力3 d后升高了3～6倍(P<0.05),并且Cbfα1的基因和蛋白表达在力学刺激下都出现上调。结论力学刺激在rBMSC的增殖与分化中起重要作用,适宜的间歇拉伸应变可以减缓BMSC的增殖,促进其成骨分化。Objective To investigate the effect of intermittent tensile strain on the proliferation and osteogenic dif- ferentiation of rBMSCs （rat bone mesenchymal stem cells）. Methods Intermittent tensile strain was applied on rBMSCs in vitro by Flexcell-4 000 Tension System （ 10% elongation amplitude, 0..5 Hz, twice every day, 4 h ev- ery time）, then effects of the strain after 1, 3, 5, 7 d on cell morphology, cell proliferation, and the relative ex- pression of Cbfcd （core binding factor al ） ,ALP and collagen I mRNA as well as Cbfal protein were measured. Results Intermittent tensile strain slowed the proliferation of rBMSCs from the first day to the seventh day. The relative expression of ALP and collagen I mRNA increased by 3 -6 times from the third day（ P 〈 0.0.5）, mean- while the expression of Cbfcd mRNA and protein was up-regulated under the mechanical stimulation. Conclusions Mechanical stretch plays an important role in the proliferation and differentiation of rBMSC, and approprite inter- mittent tensile strain can slow the proliferation of rBMSC and promot its osteogenic differentiation.

关 键 词：骨髓间充质干细胞 力学刺激 细胞培养 细胞增殖 成骨分化 

分 类 号：R783.5[医药卫生—口腔医学]