双向电泳分析重组人白细胞介素-1β对人牙髓细胞的影响  

Differential Proteomics Analysis of Dental Pulp Cells Induced by Recombinant Human Interleukin-1β

作　　者：郭世梁[1] 张颖丽[2] 张影杰[3] 柏祥娥[2] 

机构地区：[1]南京大学医学院附属口腔医院牙体牙髓科江苏南京210008 [2]吉林大学口腔医学院牙体牙髓科 [3]吉林大学中日联谊医院手术室

出　　处：《口腔医学研究》2011年第12期1029-1031,1035,共4页Journal of Oral Science Research

基　　金：吉林省科技发展计划(编号:200905178)

摘　　要：目的:观察rhIL-1β对培养的HDPCs产生蛋白质的影响,揭示rhIL-1β与牙髓炎的关系。方法:在HD-PCs的培养液中加入rhIL-1β,用2-DE分离HDPCs全蛋白,获得对照组和诱导组HDPCs蛋白质图谱,ImageMaster2DElit 5.0软件分析确认差异表达蛋白。结果:比较对照组和诱导组蛋白质图谱,发现了39个蛋白质点差异明显。其中15个蛋白质点在诱导组高表达,新增13个蛋白质点,7个蛋白质点低表达,4个蛋白质点仅在对照组中表达。结论:HDPCs对rhIL-1β的应答反应是一个非常复杂的过程,多种蛋白质分子涉及其中。Objective： To study the effects of recombinant human interleukin-1β（rhIL-1β）on the production of protein by human dental pulp cells in vitro and reveal the possible relationship between IL-1β and pulpitis.Methods： HDPCs were induced with rhIL-1β and the dental pulp cells entire protein was separated by a 2-Delectrophoresis technique.The rhIL-1β induction and the normal dental pulp cells protein 2-Delectrophoresis atlas were established.Difference expression protein was confirmed by ImageMaster 2D Elite 5.0 software analysis.Results： Comparing the two groups of protein 2-Delectrophoresis atlas,39 protein spots were obviously different.15 points in the induction of protein expression were higher,13 protein spots were new 7 protein points expressions were lower,and 4 points only were in the control group.Conclusion： Pulp cells to rhIL-1β responsiveness is a very complex process,which involve a variety of protein molecules.

关 键 词：重组人白细胞介素-1β(rhIL-1β) 双向电泳(2-DE) 蛋白质组 人牙髓细胞(HDPCs) 

分 类 号：R781.31[医药卫生—口腔医学]