山羊MyoG基因启动子区序列分析与结构预测  

Sequence Analysis and Structure Prediction of the Promoter Region of MyoG Gene in Goats

作　　者：刘铮铸[1] 巩元芳[1] 张文香[1] 付志新[1] 张传生[1] 宋瑜[2] 马艳华[3] 

机构地区：[1]河北科技师范学院动物科技学院,昌黎066600 [2]中国环境管理干部学院,秦皇岛066004 [3]河北科技师范学院欧美学院,秦皇岛066004

出　　处：《畜牧兽医学报》2012年第1期29-36,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省自然科学基金(C2007000743);国家科技支撑计划(2008BADB2B04-8-4);河北科技师范学院博士基金资助(2010YB006)

摘　　要：为探明山羊MyoG基因的启动子序列与结构及转录调控机制,本研究设计了1对引物,采用PCR扩增、测序以及序列比对分析,从波尔山羊基因组中扩增出一段长度为969bp的山羊MyoG基因的5′侧翼序列,其GC含量为50.6%。经过生物信息学分析,确定了其转录起始位点及活性区域;发现在转录起始位点上游-24bp处存在1个TATA-box,另外还发现了1个GC-box、2个CAAT-box、2个E-box和1个富含A-T碱基的模体结构;该序列还包含HSF、ADR1和cap等转录因子结合位点。通过比对分析,所得山羊MyoG基因5′侧翼序列与牛、马鹿、人、小鼠和鸡同源序列的相似性在37.22%～96.85%之间,说明不同物种间该序列具有一定的保守性。本研究为进一步探讨山羊MyoG基因的表达和调控机制奠定了理论基础。In order to explore the mechanism of goat MyoG gene transcripition regulation by studing the promoter region of the gene, the 5＇ flanking sequence of MyoG from the Bore goat genome was attained by PCR amplification, sequencing and sequence alignment. The DNA sequence of 969 bp was amplified. The content of G and C in the sequence was about 50.6%. The sequence analysis showed that there were one TATA-box at -24 bp and one GC-box, two CAAT-box, two E-box and one A＋T-rich motif. Meanwhile, some transcription factor binding sites including HSF, ADR1, cap were detected. The 5＇flanking sequence of goat MyoG was com- pared with cattle, wapiti, human, mouse and chicken, the similarity was between 37.22% and 96.85%. It indicates that the 5＇ flanking sequence of MyoG is comparatively conservative among different species. This work would lay the theoretical foundation for further study for expressing and regulating mechanism of MyoG gene in goat.

关 键 词：山羊 MYOG基因 启动子区 序列分析 结构预测 

分 类 号：S827[农业科学—畜牧学] Q523.8[农业科学—畜牧兽医]