粪肠球菌脂磷壁酸对人牙周膜成纤维细胞表达Toll样受体2及白细胞介素-1β的影响  

Study on the expression of Toll like receptor 2 and interleukin-1β induced by Enterococcus faecalis lipotei-choic acid on human periodontal ligament cells

作　　者：靳路远[1] 罗小良[1] 姜艳[1] 谢晓莉[1] 

机构地区：[1]中南大学湘雅医院口腔科,长沙410008

出　　处：《华西口腔医学杂志》2012年第1期93-96,共4页West China Journal of Stomatology

基　　金：湖南省科技厅基金资助项目(2010FJ3067)

摘　　要：目的探讨体外培养的人牙周膜成纤维细胞(HPDLCs)在粪肠球菌脂磷壁酸(LTA)刺激下表达Toll样受体2(TLR2)及白细胞介素-1β(IL-1β)的情况。方法体外分离培养HPDLCs,采用流式细胞术检测0.1、1、10μg.mL-1粪肠球菌LTA刺激24h后HPDLCs表面TLR2表达的改变,酶联免疫吸附法检测12、24、48 h后IL-1β的分泌情况,并用相同质量浓度的大肠杆菌内毒素作为对照;用TLR2中和抗体预处理HPDLCs 1 h,观察1μg.mL-1 LTA刺激24 h后其IL-1β的分泌量。结果 LTA可致HPDLCs表面TLR2表达增加(P<0.05);与对照组相比,粪肠球菌LTA可致HPDLCs分泌IL-1β增加(P<0.05),刺激12 h后可检测到IL-1β,48 h内呈上升趋势。TLR2中和抗体对粪肠球菌LTA诱导HPDLCs分泌IL-1β无明显封闭作用。结论粪肠球菌LTA可引起HPDLCs表面TLR2表达及IL-1β分泌增加。Objective To investigate the expression of Toll like receptor 2（TLR2） and interleukin-1β（IL-1β） of cultured human periodontal ligament cells （HPDLCs） activated by Enterococcus faecalis （Efaecalis） lipoteichoic acid （LTA）. Methods HPDLCs that were obtained from the periodontal tissues of healthy humans were maintained in proper condition. Flow cytometry was used to detect the expression of TLR2 on normal HPDLCs and infectious I-IPDLCs which were incubated with 0.1, 1, 10 μg.mL-1 E.faecalis LTA for 24 h. IL-1β was detected by enzyme linked immunosorbent assay （ELISA） after incubating with LTA of the above concentration for 12, 24 and 48 h or pretreated with TLR2 neutralizing antibody for 1 h and then co-cultured with 1 μg.mL-1 LTA for 24 h. Results Efaecalis LTA promoted the expression of TLR2 in normal HPDLCs. The difference had statistical significance（P〈0.05）. IL-1β secretion could be detected 12h after stimulation with LTA and increasingly escalate within 48 h（P〈0.05）. TLR2 neutralizing antibody had no evident effect on IL-1β generation stimulating by Efaeccdis LTA. Conclusion Efaecalis LTA can increase the expression of TLR2 and IL-1β in normal HPDLCs.

关 键 词：粪肠球菌 脂磷壁酸 TOLL样受体2 白细胞介素-1Β 根尖周病 

分 类 号：R781.34[医药卫生—口腔医学]