动静脉环法和动静脉束法诱导珊瑚骨血管新生的机制研究  

Mechanism of Inducing Coral Scaffold Angiogenesis by Arteriovenous Loop and Arteriovenous Bundles Model

作　　者：董青山[1] 郭家平[1] 李志进[1] 商洪涛[2] 毛天球[2] 

机构地区：[1]广州军区武汉总医院口腔科,湖北武汉430070 [2]第四军医大学口腔医学院颌面外科

出　　处：《口腔医学研究》2012年第1期26-31,共6页Journal of Oral Science Research

基　　金：湖北省自然科学基金(编号:2010CDB09206);全军"十一五"重点专项课题(06Z045)

摘　　要：目的:探讨动静脉环(AV环)法、A动静脉束(AV束)法诱导珊瑚血管新生的机制。方法:A组是将新西兰兔腘动脉和股静脉吻合成AV环,环绕套入天然珊瑚块的侧槽中加ePTFE膜包裹隔绝。B组是股动静脉血管束保持血流通畅,不切断,动静脉分开套入珊瑚块的两边侧槽中,加ePTFE膜环绕包裹隔绝。4、8周行珊瑚、血管内膜扫描电镜和血管铸型检查,同时取对侧正常股动静脉内膜作对照,血管铸型标本行大体解剖和腐蚀后血管分布等观察。结果:SEM显示A组血管化的珊瑚内部和表层有大量血管结构,相互交通,发育成熟;血管内膜凹凸不平,可见内陷发芽孔洞;B组珊瑚内血管明显偏少,血管内膜与正常内膜形态接近。血管铸型显示A组血管环周边和珊瑚表层充满小血管;B组血管明显稀疏。腐蚀铸型显示A组血管环动静脉段芽生和伴行有丰富的小血管,在入口处形成网状结构,并相互吻合交通;B组主干动脉血管无发芽新生血管,仅有周边伴行的部分小血管长入。结论:AV环方法和AV束方法均可促进珊瑚血管化,前者诱导血管新生能力更强,两者血管生成的机制完全不同。Objective： To detect the mechanism of coral angiogenesis by arteriovenous loop and arteriovenous bundle model from morphology. Methods： In group A, an arteriovenous loop （AVL） was formed by microsurgically anastomosing at the proximal ends between the femoral popliteal artery and vein, and placed in the circular side groove of coral block. In group B, a flow--through vessels bundle both femoral artery and vein （arteriovenous bundles, AVB） was placed in the side groove of coral. After 4 and 8 weeks, the cross-section of coral implant, lumen of arteriovenous vessels and contralateral femoral vessels were scanned with SEM. The rabbits were examined through abdominal aorta vascular casting. Results： SEM showed, in group A, affluent interconnecting small vessels accompanied with the large vessels both in arterial and venous segments in corals. These small vessels seemed to be mature in structure. ECs aranged randomly and unregularly. Remarkably, the vascular lumen had some minute ca- ves, both in that of artery and vein. In group B, vessels lumen ECs were spindle--shaped and arranged regularly, no invaginated lumen existed. Vascular casting showed that, in group A, significant blood vessels sprouted from all areas of the loop, especially at the entrance of the AV vascular pedicle where they were mainly interconnected dense small tubes. In group B, however, there were no blood vessels sprouted from the AV bundles and only some small vessels grown from the entrance and exit. Conclusion.. A vascularized coral model could be constructed by inserting arteriovenous loop and arteriovenous bundles, the former had more abilities to induce angiogenesis than the latter had. The mechanism of vascular regeneration in two groups was completely different.

关 键 词：血管生成 珊瑚 动静脉短路环 动静脉血管束 

分 类 号：R782[医药卫生—口腔医学]