肥胖大鼠袖状胃切除术模型的建立  

Establishment of sleeve gastrectomy model in diet-induced obese Sprague-Dawley rats

作　　者：马冠君[1] 张伟[1] 郑向民[1] 仇明[1] 

机构地区：[1]第二军医大学附属长征医院普通外科,上海200003

出　　处：《中华胃肠外科杂志》2012年第1期43-46,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的探讨肥胖大鼠袖状胃切除术动物模型的建立方法，用于研究降糖机制。方法9只6周龄雄性Spraglle-Dawlev大鼠喂养高脂饲料4周制备成单纯营养性肥胖大鼠后．被随机分为2组，6只行袖状胃切除术[袖状胃组，体质量（471．8±17．9）g]，3只行胃干预手术[假手术组，体质量（467．0±8．4）g]。术后测量大鼠体质量、摄食量和空腹血浆ghrelin浓度变化。结果两组大鼠术后早期体质量均进行性下降．假手术组术后第5天体质量开始回升．第22天反弹至术前水平；而袖状胃组术后第9天体质量开始缓慢回升，术后第28天体质量[（420．1±18．6）g]仍明显低于同组术前水平[（471．8±17．9）g]及假手术组[（487．4±10．1）g，P=0．001]。术后袖状胃组大鼠平均每日摄食量较假手术组明显减少，但两组差异无统计学意义（P=0．121）。袖状胃组术后血浆总ghrelin浓度呈连续下降趋势，术后4周[（1316．8±14．8）ng／L]与术前[（1595．1±14．4）ng／L]相比下降17．4％；假手术组手术前后无明显变化，两组差异有统计学意义（P=0．000）。结论成功建立的大鼠外科袖状胃切除术模型可应用来研究2型糖尿病袖状胃切除术后改善糖代谢的机制。Objective To establish sleeve gastrectomy （SG） rats model of obese type 2 diabetes mellitus （T2DM） for the research of hypoglycemic mechanism. Methods Nine male Sprague-Dawley （6-week-old） rats were fed with high-sucrose and high-fat diet for 4 weeks, developing diet-induced obesity （DIO） rats model. The rats were then randomly divided into two groups. Six rats of them underwent sleeve gastrectomy （SG） as the sleeve gastrectomy group [ SGG, body weight （471.8 ＋ 17.9 ） g ] and the other three rats underwent a laparotomy and stomach manipulation as the sham operative group [SOG, body weight （467.0±8.4） g]. The body weight, caloric intake and peripheral blood concentration of total ghrelin of rats were recorded after operation. Results The weight of all the rats declined progressively after operation. The weight of the rats in SOG began to rise on the 5th postoperative day （POD） and regain their preoperative levels on the average 22nd POD. However, the weight of the rats in SGG began to rise slowly from the 9th POD, but was still lower than that of SOG[（487.4±10.1） g] and preoperative levels [（471.8±17.9） g] on the 28th POD [（420.1±18.6） g] （P=0.001）. Average caloric intake of rats in SGG was significantly lower than that of SOG after operation, but there was no significant difference between the two groups （P=0.121）. The ghrelin level of SGG showed a continuous decreasing trend after intervention, decreased by 17.4% compared with the preoperative level （1595.1±14.4 ng/L） on the 28th POD [ （1316.8±14.8） ng/L]. The ghrelin level of SOG did not change obviously before and after operation and both groups differred statistically（P=0.000）. Conclusions A SG rat model is successfully established. This model can be used for the further study of mechanism analysis of T2DM resolution after surgery.

关 键 词：袖状胃切除术 大鼠模型 肥胖 GHRELIN 

分 类 号：R735.2[医药卫生—肿瘤]