中国朝鲜族六个Y染色体短串联重复基因座遗传多态性的研究  

Genetic polymorphisms of six Y chromosome short tandem repeat loci in Chinese Korean ethnic group

作　　者：张永吉[1] 李松花[1] 段洪瑞[1] 

机构地区：[1]延吉市,延边大学基础医学院法医学教研室,吉林省133002

出　　处：《中华医学遗传学杂志》2012年第1期82-86,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30660201）

摘　　要：目的检测吉林延边地区朝鲜族人群6个Y染色体短串联重复（Y-short tandem repeat，Y-STR）基因座DYS441、DYS442、DYS443、DYS444、DYS445和DYS446的遗传多态性，以获取相应的遗传多态性数据。方法应用PCR扩增、变性聚丙烯酰胺凝胶电泳和银染法对205名延边地区朝鲜族男性个体的6个Y-STR基因座进行检测和分析。结果在6个基因位点中，分别检出了8、7、7、5、6和9个等位基因，基因多样性分布为0．5302-0．7897。共发现151种单倍型，单倍型基因多样性为0．9938。结论6个Y-STR基因座在延边地区朝鲜族群体中均呈现较强的遗传多态性，因此是较为理想的遗传标记系统，在父系遗传关系确定和中国朝鲜族起源研究等方面具有较高的应用价值。Objective To determine the allelic sequences and genetic polymorphism of six short tandem repeats （STRs） loci on Y chromosome, including DYS441, DYS442, DYS443, DYS444, DYS445 and DYS446 in Chinese Korean ethnic males from Yanbian region of Jilin province, China, and to construct a preliminary database. Methods Allele frequencies of the six STR loci in 205 Chinese Korean ethnic individuals were analyzed by multiplex polymerase chain reaction （PCR） and polyacrylamide gel electrophoresis （PAGE）. Results Respectively, 8, 7, 7, 5, 6 and 9 alleles were detected for each of the locus. Together they have formed 151 haplotypes, with a diversity of 0. 9937. Conclusion The six STR loci included in this study were found to be highly polymorphic, and may provide useful markers for genetic analysis.

关 键 词：朝鲜族 Y染色体短串联重复 遗传多态性 法医学鉴定 

分 类 号：R394[医药卫生—医学遗传学]