立体分子伴侣-脂肪酶特异折叠酶  

Steric Chaperone-lipase Specific Foldase

作　　者：郑小梅[1] 初晓宇[1] 伍宁丰[1] 范云六[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2011年第6期66-71,共6页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30900839)资助

摘　　要：细菌脂肪酶是一类重要的工业用酶,尤其以来源于假单孢杆菌Pseudomonas与伯克霍尔德菌Burk-holderia的脂肪酶应用最为广泛。然而,这类脂肪酶折叠过程中存在一个巨大的能障而无法自发正确折叠。研究发现,这类脂肪酶的编码基因所在操纵子中往往存在一个与其折叠相关的基因,编码一种脂肪酶特异折叠酶。该类蛋白为脂肪酶的立体分子伴侣,可与脂肪酶的折叠中间体相互作用,帮助脂肪酶越过折叠过程中的能量障碍,为其获得正确构象提供必要的空间信息。详细阐述了脂肪酶特异折叠酶的发现、分类、作用机制与应用前景。Lipases represent the most important class of enzymes used in industry. The enzymes originated fi＇om Pseudomonas and Burkholderia species are wildly used. But a huge energetic barrier traps kinetically disable lipases in folding state to fold rightly. Research found that these enzymes were usually encoded in an operon together with a second gene, which coded for a lipase-specific-foldase, Lif. It is necessary for obtaining enzymatically active lipase. Lifs are so-called sterie chaperones, which exert their physiological function by lowering energetic barriers when folding their cognate lipases, thereby providing essential steric information needed for folding lipases into their enzymatically active conformation. In this review, we elaborated the discovery, classification, mechanism, and perspective for application of lipase specificfoldase.

关 键 词：细菌脂肪酶 脂肪酶特异折叠酶 立体分子伴侣 蛋白折叠 

分 类 号：Q55[生物学—生物化学]