Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine  

作　　者：ZHANG XiZhen WANG XiaoDan ZHAO DongHai MENG XiangYu ZHAO XingHong YU XiangHui KONG Wei 

机构地区：[1]National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, China [2]Key Laboratoryfor Molecular Enzymology and Engineering of Ministry of Education of China, College of Life Science, Jilin University, Changchun 130012, China [3]jilin Medical College, Jilin 132013, China

出　　处：《Science China(Life Sciences)》2011年第11期1042-1047,共6页中国科学（生命科学英文版）

基　　金：supported by the Na-tional Natural Science Foundation of China (Grant No. 30371317) to Kong Wei

摘　　要：The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine.Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns,HIV virus like particles(VLPs) offer an attractive alternative because they are considered safer since they lack viral genome.We got a stable eukaryotic cell line by G418 resistance selection,engineered to express the HIV-1 structure protein Gag and Env efficiently and stably.We confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into VLPs.These VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant.The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine.Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns,HIV virus like particles（VLPs） offer an attractive alternative because they are considered safer since they lack viral genome.We got a stable eukaryotic cell line by G418 resistance selection,engineered to express the HIV-1 structure protein Gag and Env efficiently and stably.We confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into VLPs.These VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant.

关 键 词：HIV-1 COTRANSFECTION stable cell line virus-like particles（VLPs） VACCINE 

分 类 号：S944.47[农业科学—水产养殖] Q78[农业科学—水产科学]