5-烯丙基-7-二氟亚甲氧白杨素体外抗肝癌作用  

Antihepatoma effect of 5-ally-7-gen-difluormethylenechrysin on the proliferation and apoptosis of human liver cancer

作　　者：王宇[1] 张亮[2] 周建国[2] 周秀田[2] 

机构地区：[1]长治医学院病理教研室 [2]南华大学肿瘤研究所

出　　处：《齐齐哈尔医学院学报》2011年第22期3606-3607,共2页Journal of Qiqihar Medical University

基　　金：湖南省医药卫生科研基金(B2006-102)

摘　　要：目的研究白杨素(ChR)衍生物5-烯丙基-7-二氟亚甲氧白杨素(5-ally-7-gen-difluormethylenechrysin,ADFMChR)对体外培养肝癌SMMC-7721细胞生长抑制作用及诱导凋亡的作用机制。方法 1)细胞计数法研究ADFMChR对SMMC-7721细胞生长曲线及细胞倍增时间的影响;2)Western blot法分析ADFMChR对SMMC-7721细胞Bcl-2、Bax蛋白表达变化情况。结果 1)细胞计数结果表明:0.3μM～30.0μM浓度的ADFMChR与对照组相比均可抑制SMMC-7721细胞生长,且呈浓度和时间依赖性;常规培养SMMC-7721细胞群体倍增时间为23.81小时,当ADFMChR为30.0μM时则可使其延长至61.22小时;2)Western Blot分析证实ADFMChR上调Bax和下调Bcl-2呈浓度依赖性。结论以ChR为先导物,选择性引入烯丙基和二氟亚甲氧基的衍生物ADFMCHR对肝癌的生长抑制作用较ChR明显增强,可能与细胞增殖周期延长,Bcl-2/Bax比值下降,诱导细胞凋亡相关。Objective To investigate the effect and mechanism of 5-ally-7-gen-difluormethylene chrysin（ADFMChR）,a novel chrysin derivatives,on the proliferation and apoptosis of human liver cancer（SMMC-7721） cell line in vitro.Methods SMMC-7721cells was cultured in vitro.Cell counting method was used to test the influence of growth curve in SMMC-7721 cells treated with different concentrations of ADFMChR.Western blot analyzed the expression of Bcl-2,Bax proteins.Results Cell counting showed that average doubling time retarded from 23.81h in normal culture SMMC-7721 cells to 61.22 h in 30.0uM ADFMChR experimented SMMC-7721 cells.Western blot analysis indicated that ADFMChR could down-regulate expression of Bcl-2 proteins,meanwhile,up-regulate expression of Bax protein in SMMC-7721 cells.Conclusions After modification by introducing ally and gen-difluormethylen in chrysin,the molecular mechanisms of ADFMChR inhibited proliferation and induced apoptosis of SMMC-7721 cells may be associated with doubling time retarded and down regulation the value of Bcl-2 /Bax protein.

关 键 词：肝癌 5-烯丙基-7-二氟亚甲基白杨素 细胞增殖周期 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]