国产荧光原位杂交探针检测自然流产绒毛染色体的临床应用研究  

Clinical application and study of domestic fluorescence in situ hybridization in detection of chromosome in chorionic tissue of cases with spontaneous abortion

作　　者：胡红霞[1] 丛林[1] 袁静[1] 赵保静[1] 方慧琴[1] 陈薇[1] 

机构地区：[1]安徽医科大学第一附属医院妇产科产前诊断中心,安徽合肥230022

出　　处：《中国妇幼保健》2011年第36期5804-5807,共4页Maternal and Child Health Care of China

基　　金：中华医学会分子生物学临床应用研究专项资金项目〔CAMB012010〕

摘　　要：目的:应用国产荧光原位杂交(FISH)探针,对自然流产绒毛组织进行遗传学分析。方法:收集自然妊娠后自然流产绒毛80例,经体外受精-胚胎移植(IVF-ET)治疗后自然流产绒毛20例,行荧光免疫杂交(fluorescence in situ hy-bridization,FISH)技术分析流产绒毛13、16、18、21、22、X和Y这5对常染色体和1对性染色体的非整倍体状况,并与细胞培养核型分析技术相对比。结果:FISH技术共检测出30例染色体异常,细胞培养核型分析共检测出38例染色体异常;自然流产组与IVF-ET流产组的染色体异常率分别为38.75%(31/80)、35.00%(7/20),差异无统计学意义(P>0.05);在80例自然妊娠后自然流产病例中,既往0、1、2、3次及以上自然流产史的染色体异常率分别为28.30%(15/53)、30.00%(3/10)、30.00%(3/10)、33.33%(1/3),各组间比较无统计学差异;80例自然妊娠自然流产病例中,年龄在35岁及以下和大于35岁者染色体异常率分别为38.23%(26/68)、41.67%(5/12),IVF-ET组分别为21.43%(3/14)、66.67%(4/6),差异均无统计学意义(P>0.05)。结论:自然妊娠后自然流产与IVF-ET术后自然流产的发生均与染色体异常密切相关,其染色体异常率无明显差异。Objective：To carry out genetic analysis of chorionic tissue of cases with spontaneous abortion by using domestic fluorescence in situ hybridization（FISH） probe.Methods：80 samples of chorionic tissue after spontaneous abortion and 20 samples of chorionic tissue after spontaneous abortion treated with in vitro fertilization-embryo transfer（IVF-ET） were collected,FISH technique was used to analyze the heteroploid conditions of five pairs of autosome（13,16,18,21,22） and one pair of sex chromosome（X and Y）,then the results were compared with cell culture karyotype analysis technique.Results：30 cases with chromosomal abnormality were detected by FISH technique,and 38 cases with chromosomal abnormality were detected by cell culture karyotype analysis technique;the incidences of chromosomal abnormality in spontaneous abortion group and IVF-ET abortion group were 38.75%（31/80） and 35.00%（7/20）,respectively,there was no significant difference between the two groups（P0.05）.In spontaneous abortion group,the incidences of chromosomal abnormality in cases without the history of spontaneous abortion,the cases with the histories of spontaneous abortion for once,twice,three times and more than three times were 28.30%（15/53）,30.00%（3/10）,30.00%（3/10） and 33.33%（1/3）,respectively,there was no significant difference among different groups;in spontaneous abortion group,the incidences of chromosomal abnormality in cases less than 35 years and more than 35 years were 38.23%（26/68） and 41.67%（5/12）,respectively;in IVF-ET abortion group,the incidences of chromosomal abnormality in cases less than 35 years and more than 35 years were 21.43%（3/14） and 66.67%（4/6）,respectively,in the two groups,there was no significant difference between the two age groups（P0.05）.Conclusion：The occurrence of spontaneous abortion after natural pregnancy and IVF-ET are both related to chromosomal abnormality closely,but there is no significant difference in the incidence of chromosomal ab

关 键 词：荧光原位杂交技术 体外受精-胚胎移植 自然流产 绒毛染色体 

分 类 号：R714.21[医药卫生—妇产科学]