Determination of levels of adenosine phosphates in blood by ion chromatography  

作　　者：Pei Jin Xie Ming Li Ye Zhong Yang Hu Guang Wen Pan Yan Zhu Jia Jie Zhang 

机构地区：[1]Department of Chemistry,Zhejiang University,Hangzhou 310028,China [2]Shanghai Lab,Dionex China Co.,Ltd.,Shanghai 201203,China

出　　处：《Chinese Chemical Letters》2011年第12期1485-1488,共4页中国化学快报（英文版）

基　　金：financially supported by National Natural Science Foundation of China(Nos.20775070 and J0830413);Zhejiang Provincial Natural Science Foundation of China(Nos.R4080124 and Y4090268)

摘　　要：A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.

关 键 词：Adenosine phosphate Ion chromatography BLOOD 

分 类 号：O657.32[理学—分析化学] S816.3[理学—化学]