簇集蛋白在乳腺浸润性导管癌中的表达及其临床意义  

Expression and clinical significance of Clusterin protein in invasive ductal breast cancer

作　　者：李景武[1] 张景华[1] 薛书霞[1] 张志勇[2] 

机构地区：[1]河北省唐山市人民医院肿瘤外科,063001 [2]唐山市工人医院病理科

出　　处：《肿瘤研究与临床》2011年第12期807-809,816,共4页Cancer Research and Clinic

基　　金：唐山市市级科研课题（10130298c）

摘　　要：目的探讨细胞凋亡抑制因子簇集蛋白在乳腺浸润性导管癌组织中的表达及临床意义。方法应用免疫组织化学sP法检测70例乳腺浸润性导管癌、20例乳腺增生和10例乳腺癌旁组织（距癌组织1〉4cm，组织学结构正常）标本中簇集蛋白的表达情况，并探讨其与乳腺癌各临床病理学参数间的关系。结果簇集蛋白在乳腺癌旁正常组织、乳腺增生及乳腺浸润性导管癌组织中的阳性表达率分别为0、20．0％（4／20）及71．4％（50／70），乳腺浸润性导管癌组织中的簇集蛋白表达水平明显高于乳腺增生（χ2=17．143，P〈O．05）及乳腺癌旁正常组织（χ2=19．048，／9〈0．05）；簇集蛋白在乳腺浸润性导管癌中的表达随着临床分期的增加阳性表达率显著升高（χ2=4．667，P〈0．05），且与组织学分级及淋巴结转移情况有关（χ2=5．233，P〈0．05；χ2=4．667，P〈0．05），与患者年龄、肿瘤大小及组织学类型无关（χ2值分别为0．024、0．406、0．091，均／9〉0．05）。在乳腺浸润性导管癌组织中簇集蛋白与ER、PR的表达呈负相关（r值分别为-0．362、-0．290，均／9〈O．05），与C—erbB-2的表达无相关性（r=0．129，P〉0．05）。结论簇集蛋白可能通过抑制细胞凋亡在乳腺癌的发生、发展中发挥促进作用，可能成为乳腺浸润性导管癌诊断中的标志物，并有望成为乳腺癌治疗的新靶点。Objective To investigate the expression and the clinical significance of Clusterin protein in invasive ductal breast cancer. Methods The expression of Clusterin protein in 70 cases of invasive ductal breast cancer, 20 cases of breast hyperplasia, and 10 cases of adjacent normal breast tissue was examined with SP immunohistochemistry （IHC）, and their correlation with some cfinicopathological parameters were studied. Results Clusterin expression was detected in 71.4 % of breast cancer tissues, which was significantly higher than that in benign lesions （20.0 %） and adjacent normal tissues （0） （χ2 = 17.143, P 〈 0.05; χ2 = 19.048, P 〈 0.05）. Clusterin expression in breast cancer was relevant to the histological grade （χ2 = 5.233, P 〈 0.05）, lymph node metastasis （χ2 = 4.677, P 〈 0.05）, and the progression in the clinical stage （χ2 = 4.667, P 〈 0.05）, but irrelevant to the age （χ2=- 0.024, P 〉 0.05） and tumor size （χ2= 0.406, P 〉 0.05）. Clusterin expression was negatively correlated to the expression of ER and PR in breast cancer tissues （r = -0.362, P 〈 0.05 and r = -0.290, P 〈 0.05, respectively）, but uncorrelated with C-erbB-2 expression （r = 0.129, P 〉 0.05）. Conclusion Clusterin overexpressed in breast cancer tissues suggests an important role of clusterin in invasive ductal breast cancer carcinogenesis and development through inhibition of cell apoptosis. It may become new clinical diagnosis marker for breast cancer and novel target for breast cancer therapy.

关 键 词：癌 导管 乳腺 簇集蛋白 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]