2-甲氧基雌二醇对全脑缺血大鼠HIF-1α及RTP801的作用研究  

Effects of 2ME2 on hypoxia-inducible factor 1α and apoptosis-related gene RTP801 in hippocampus following the global cerebral ischemia in rats

作　　者：彭静华[1] 张鸿日[2] 黄丽娜[1] 李云[3] 

机构地区：[1]河南科技大学第一附属医院神经内科,河南洛阳471003 [2]河南科技大学第一附属医院神经外科,河南洛阳471003 [3]山东大学附属济南市中心医院急诊科,山东济南250013

出　　处：《中国现代医生》2012年第1期6-8,共3页China Modern Doctor

基　　金：山东省济南市科学技术发展计划项目(051082)

摘　　要：目的探讨2-甲氧基雌二醇(2ME2)对全脑缺血大鼠HIF-1α及凋亡相关基因RTP801的作用。方法将成年雄性SD大鼠104只随机分为对照组(n=8)、全脑缺血组(n=48)和全脑缺血2-甲氧基雌二醇干预组(n=48),后两组按再灌注时间不同各分为6个亚组。采用改良的Pulsineli 4-VO方法行全脑缺血大鼠模型制作。Nissl染色进行海马神经元计数,免疫组化及RT-PCR技术进行HIF-1α及RTP801 mRNA表达检测。结果全脑缺血2ME2干预组与全脑缺血组相应时间点亚组相比,神经细胞计数明显增加,HIF-1α表达减少,RTP801 mRNA表达减低(P<0.05)。结论在全脑缺血急性期,2ME2抑制HIF-1α及凋亡相关基因RTP801表达,具有一定的神经保护作用。Objective To investigate the effect of 2ME2 on the hypoxia-inducible factor 1α and apoptosis-related gene RTP801 i, hippoeampus following the global cerebral ischemia in rats. Methods One hundred and four adult male Sprague-Dawley rats were randomly divided into 3 groups： sham group （n=8）, global cerebral isehemia group （n=48） and global cerebral ischemia ＋ 2ME2 treatment group （n=48）, and the rats of GI group and GI ＋ 2ME2 group were sacrificed after perfusion. The 4 vessel occlusion （4-VO） global isehemia rat model was produced. Nissl staining was used to perform quantitative analysis of cell numbers. Immunohistochem- istry staining and RT-PCR provided the information of the distribution of positive cells to HIF-lot protein and the expression of RTP80] mRNA. Results The number of cells from GI ＋ 2ME2 group was significantly higher than that from GI group （P〈0.05）. 2ME2 significantly suppressed the levels of HIF-1α （/）〈0.05）. The expression of apoptosis-related gene RTPS01 decreased significantly （P〈0.05）. Conclusion 2ME2 has brain protective effect on global cerebral ischemia in rats. 2ME2 inhibits the elevation of hypoxia- indueible factor 1α, which inhibits the elevation of apoptosis-related gene RTP801.

关 键 词：RTP801 全脑缺血 缺氧诱导因子-1 2-甲氧基雌二醇 

分 类 号：R743.31[医药卫生—神经病学与精神病学]